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Alternative name
Insulin-like growth factor-binding protein 7 ELISA KIT; IGFBP-rP1 ELISA KIT; MAC25 protein ELISA KIT; PGI2-stimulating factor ELISA KIT; Prostacyclin-stimulating factor ELISA KIT; Tumor-derived adhesion factor ELISA KIT; TAFIGFBP7 ELISA KIT; MAC25 ELISA KIT; PSF ELISA KIT; IBP-7 ELISA KIT; IGF-binding protein 7 ELISA KIT; IGFBP-7 ELISA KIT; TAF ELISA KIT
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Catalog
E056828
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species
Mouse
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GeneIGFBP-7
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<46.9pg/ml
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Intended UseMouse IGFBP-7 ELISA Kit allows for the in vitro quantitative determination of IGFBP-7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse IGFBP-7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse IGFBP-7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IGFBP-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse IGFBP-7 can be calculated.
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