Human NUP160 ELISA Kit

Alternative name
Human 160 kDa nucleoporin ELISA Kit;Human nucleoporin Nup160 ELISA Kit;Human KIAA0197 ELISA Kit;Human NUP120 ELISA Kit;Human nucleoporin 160 ELISA Kit;Human nuclear pore complex protein Nup160 ELISA Kit;Human nucleoporin 160kD ELISA Kit;Human nucleoporin 160kDa ELISA Kit;
Catalog
E027091
species
Human
GeneNUP160
Standard Curve
Other Species Mouse Nup160 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of NUP160. No significant cross-reactivity or interference between NUP160 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NUP160 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman NUP160 ELISA Kit allows for the in vitro quantitative determination of NUP160 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This NUP160 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NUP160. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||NUP160 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NUP160 antibody and an NUP160-HRP conjugate. The assay sample and buffer are incubated together with NUP160-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP160 concentration since NUP160 from samples and NUP160-HRP conjugate compete for the anti-NUP160 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP160 from the sample, fewer sites are left to bind NUP160-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP160 concentration in each sample is interpolated from this standard curve.
Human Nuclear pore complex protein Nup160 Protein information
Uniprot ID NU160_HUMAN
Uniprot AC Q12769; B4DYE8; B4E2J9; Q08AD3; Q7Z5X6; Q96GB3; Q9H660;
UniGene Hs.643526; Hs.744107;
GeneID 23279
KEGG hsa:23279;
Human Nuclear pore complex protein Nup160 Protein SEQUENCE
SEQUENCE 1436 AA; 162121 MW; 274C063A3CE69F48 CRC64;
MLHLSAAPPA PPPEVTATAR PCLCSVGRRG DGGKMAAAGA LERSFVELSG
AERERPRHFR EFTVCSIGTA NAVAGAVKYS ESAGGFYYVE SGKLFSVTRN
RFIHWKTSGD TLELMEESLD INLLNNAIRL KFQNCSVLPG GVYVSETQNR
VIILMLTNQT VHRLLLPHPS RMYRSELVVD SQMQSIFTDI GKVDFTDPCN
YQLIPAVPGI SPNSTASTAW LSSDGEALFA LPCASGGIFV LKLPPYDIPG
MVSVVELKQS SVMQRLLTGW MPTAIRGDQS PSDRPLSLAV HCVEHDAFIF
ALCQDHKLRM WSYKEQMCLM VADMLEYVPV KKDLRLTAGT GHKLRLAYSP
TMGLYLGIYM HAPKRGQFCI FQLVSTESNR YSLDHISSLF TSQETLIDFA
LTSTDIWALW HDAENQTVVK YINFEHNVAG QWNPVFMQPL PEEEIVIRDD
QDPREMYLQS LFTPGQFTNE ALCKALQIFC RGTERNLDLS WSELKKEVTL
AVENELQGSV TEYEFSQEEF RNLQQEFWCK FYACCLQYQE ALSHPLALHL
NPHTNMVCLL KKGYLSFLIP SSLVDHLYLL PYENLLTEDE TTISDDVDIA
RDVICLIKCL RLIEESVTVD MSVIMEMSCY NLQSPEKAAE QILEDMITID
VENVMEDICS KLQEIRNPIH AIGLLIREMD YETEVEMEKG FNPAQPLNIR
MNLTQLYGSN TAGYIVCRGV HKIASTRFLI CRDLLILQQL LMRLGDAVIW
GTGQLFQAQQ DLLHRTAPLL LSYYLIKWGS ECLATDVPLD TLESNLQHLS
VLELTDSGAL MANRFVSSPQ TIVELFFQEV ARKHIISHLF SQPKAPLSQT
GLNWPEMITA ITSYLLQLLW PSNPGCLFLE CLMGNCQYVQ LQDYIQLLHP
WCQVNVGSCR FMLGRCYLVT GEGQKALECF CQAASEVGKE EFLDRLIRSE
DGEIVSTPRL QYYDKVLRLL DVIGLPELVI QLATSAITEA GDDWKSQATL
RTCIFKHHLD LGHNSQAYEA LTQIPDSSRQ LDCLRQLVVV LCERSQLQDL
VEFPYVNLHN EVVGIIESRA RAVDLMTHNY YELLYAFHIY RHNYRKAGTV
MFEYGMRLGR EVRTLRGLEK QGNCYLAALN CLRLIRPEYA WIVQPVSGAV
YDRPGASPKR NHDGECTAAP TNRQIEILEL EDLEKECSLA RIRLTLAQHD
PSAVAVAGSS SAEEMVTLLV QAGLFDTAIS LCQTFKLPLT PVFEGLAFKC
IKLQFGGEAA QAEAWAWLAA NQLSSVITTK ESSATDEAWR LLSTYLERYK
VQNNLYHHCV INKLLSHGVP LPNWLINSYK KVDAAELLRL YLNYDLLEEA
VDLVSEYVDA VLGKGHQYFG IEFPLSATAP MVWLPYSSID QLLQALGENS
ANSHNIALSQ KILDKLEDYQ QKVDKATRDL LYRRTL
UCSC uc001ngm.4; human. [Q12769-1];

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Human NUP160 ELISA Kit

Human 160 kDa nucleoporin ELISA Kit;Human nucleoporin Nup160 ELISA Kit;Human KIAA0197 ELISA Kit;Human NUP120 ELISA Kit;Human nucleoporin 160 ELISA Kit;Human nuclear pore complex protein Nup160 ELISA Kit;Human nucleoporin 160kD ELISA Kit;Human nucleoporin 160kDa ELISA Kit;