Human NF-kappaB p65 ELISA Kit

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  • Alternative name

    Nuclear factor NF-kappa-B p100 subunit ELISA KIT; DNA-binding factor KBF2 ELISA KIT; H2TF1 ELISA KIT; Lymphocyte translocation chromosome 10 protein ELISA KIT; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 ELISA KIT; Oncogene Lyt-10 ELISA KIT; Lyt10NFKB2 ELISA KIT; LYT10 ELISA KIT; Lyt10 ELISA KIT

  • Catalog
    E026888
  • species
    Human
  • GeneNF-kappaB p65
  • Other Species Mouse NF-kappaB p65 ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity12 pg/ml.
  • Intended UseHuman NF-kappaB p65 ELISA Kit allows for the in vitro quantitative determination of NF-kappaB p65 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human NF-kappaB p65 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.




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