Human CANCA ELISA Kit

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  • Alternative name

    Caspase-1 ELISA KIT; Interleukin-1 beta convertase ELISA KIT; IL-1BC ELISA KIT; Interleukin-1 beta-converting enzyme ELISA KIT; ICE ELISA KIT; IL-1 beta-converting enzyme ELISA KIT; p45Cleaved into the following 2 chains:Caspase-1 subunit p20 ELISA KIT; Caspase-1 subunit p10CASP1 ELISA KIT; IL1BC ELISA KIT; CASP-1 ELISA KIT; IL-1BC ELISA KIT; ICE ELISA KIT; IL-1 beta-converting enzyme ELISA KIT

  • Catalog
    E002645
  • species
    Human
  • GeneCANCA
  • Standard CurveHuman CANCA ELISA Kit
  • Other Species Mouse CANCA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CANCA ELISA Kit allows for the in vitro quantitative determination of CANCA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: ICE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ICE. Standards or samples are then added to the microtiter plate wells and ICE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ICE present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ICE are added to each well to "sandwich" the ICE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ICE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICE concentration in each sample is interpolated from this standard curve.




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