Human IDH2 ELISA Kit

Alternative name
Human IDH ELISA Kit;Human ICD-M ELISA Kit;Human IDP ELISA Kit;Human NADP(+)-specific ICDH ELISA Kit;Human oxalosuccinate decarboxylase ELISA Kit;Human D2HGA2 ELISA Kit;Human IDHM ELISA Kit;Human IDPM ELISA Kit;Human mNADP-IDH ELISA Kit;Human isocitrate dehydrogenase (NADP(+)) 2, mitochondrial ELISA Kit;Human isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit;Human isocitrate dehydrogenase 2 (NADP+), mitochondrial ELISA Kit;
Catalog
E021122
species
Human
GeneIDH2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 2, mitochondrial (IDH2). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 2, mitochondrial (IDH2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.136ng/mL.
Intended UseHuman IDH2 ELISA Kit allows for the in vitro quantitative determination of IDH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 2, mitochondrial (IDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 2, mitochondrial (IDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isocitrate Dehydrogenase 2, mitochondrial (IDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isocitrate Dehydrogenase 2, mitochondrial (IDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Isocitrate dehydrogenase [NADP], mitochondrial Protein information
Uniprot ID IDHP_HUMAN
Uniprot AC P48735; B2R6L6; B4DFL2; Q96GT3;
UniGene Hs.596461;
GeneID 3418
KEGG hsa:3418;
Human Isocitrate dehydrogenase [NADP], mitochondrial Protein SEQUENCE
SEQUENCE 452 AA; 50909 MW; 4DDC830AFC06AB52 CRC64;
MAGYLRVVRS LCRASGSRPA WAPAALTAPT SQEQPRRHYA DKRIKVAKPV
VEMDGDEMTR IIWQFIKEKL ILPHVDIQLK YFDLGLPNRD QTDDQVTIDS
ALATQKYSVA VKCATITPDE ARVEEFKLKK MWKSPNGTIR NILGGTVFRE
PIICKNIPRL VPGWTKPITI GRHAHGDQYK ATDFVADRAG TFKMVFTPKD
GSGVKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYAI QKKWPLYMST
KNTILKAYDG RFKDIFQEIF DKHYKTDFDK NKIWYEHRLI DDMVAQVLKS
SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT
VTRHYREHQK GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQMLEK
VCVETVESGA MTKDLAGCIH GLSNVKLNEH FLNTTDFLDT IKSNLDRALG
RQ
UCSC uc002box.4; human. [P48735-1];

Category

Human IDH2 ELISA Kit