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Mouse F8 ELISA Kit

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  • Alternative name

    Mouse procoagulant component ELISA Kit;Mouse Cf8 ELISA Kit;Mouse F8c ELISA Kit;Mouse Cf-8 ELISA Kit;Mouse FVIII ELISA Kit;Mouse coagulation factor VIII ELISA Kit;Mouse Factor VIII ELISA Kit;
  • Catalog
    E048788
  • species
    Mouse
  • GeneF8
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of F8. No significant cross-reactivity or interference between F8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between F8 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse F8 ELISA Kit allows for the in vitro quantitative determination of F8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical    Principle of the Assay: F8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F8 antibody and an F8-HRP conjugate. The assay sample and buffer are incubated together with F8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F8 concentration since F8 from samples and F8-HRP conjugate compete for the anti-F8 antibody binding site. Since the number of sites is limited, as more sites are occupied by F8 from the sample, fewer sites are left to bind F8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F8 concentration in each sample is interpolated from this standard curve.
  • Mouse Coagulation factor VIII Protein information
  • Uniprot ID FA8_MOUSE
  • Uniprot AC Q06194; A2AN88;
  • UniGene Mm.1805;
  • GeneID 14069
  • KEGG mmu:14069;
  • Mouse Coagulation factor VIII Protein SEQUENCE

  • SEQUENCE 2319 AA; 266196 MW; 09CAC4CFBCCECA57 CRC64;

    MQIALFACFF LSLFNFCSSA IRRYYLGAVE LSWNYIQSDL LSVLHTDSRF

    LPRMSTSFPF NTSIMYKKTV FVEYKDQLFN IAKPRPPWMG LLGPTIWTEV

    HDTVVITLKN MASHPVSLHA VGVSYWKASE GDEYEDQTSQ MEKEDDKVFP

    GESHTYVWQV LKENGPMASD PPCLTYSYMS HVDLVKDLNS GLIGALLVCK

    EGSLSKERTQ MLYQFVLLFA VFDEGKSWHS ETNDSYTQSM DSASARDWPK

    MHTVNGYVNR SLPGLIGCHR KSVYWHVIGM GTTPEIHSIF LEGHTFFVRN

    HRQASLEISP ITFLTAQTLL IDLGQFLLFC HISSHKHDGM EAYVKVDSCP

    EESQWQKKNN NEEMEDYDDD LYSEMDMFTL DYDSSPFIQI RSVAKKYPKT

    WIHYISAEEE DWDYAPSVPT SDNGSYKSQY LSNGPHRIGR KYKKVRFIAY

    TDETFKTRET IQHESGLLGP LLYGEVGDTL LIIFKNQASR PYNIYPHGIT

    DVSPLHARRL PRGIKHVKDL PIHPGEIFKY KWTVTVEDGP TKSDPRCLTR

    YYSSFINPER DLASGLIGPL LICYKESVDQ RGNQMMSDKR NVILFSIFDE

    NQSWYITENM QRFLPNAAKT QPQDPGFQAS NIMHSINGYV FDSLELTVCL

    HEVAYWHILS VGAQTDFLSI FFSGYTFKHK MVYEDTLTLF PFSGETVFMS

    MENPGLWVLG CHNSDFRKRG MTALLKVSSC DKSTSDYYEE IYEDIPTQLV

    NENNVIDPRS FFQNTNHPNT RKKKFKDSTI PKNDMEKIEP QFEEIAEMLK

    VQSVSVSDML MLLGQSHPTP HGLFLSDGQE AIYEAIHDDH SPNAIDSNEG

    PSKVTQLRPE SHHSEKIVFT PQPGLQLRSN KSLETTIEVK WKKLGLQVSS

    LPSNLMTTTI LSDNLKATFE KTDSSGFPDM PVHSSSKLST TAFGKKAYSL

    VGSHVPLNVS EENSDSNILD STLMYSQESL PRDNILSMEN DRLLREKRFH

    GIALLTKDNT LFKDNVSLMK TNKTYNHSTT NEKLHTESPT SIENSTTDLQ

    DAILKVNSEI QEVTALIHDG TLLGKNSTYL RLNHMLNRTT STKNKDIFHR

    KDEDPIPQDE ENTIMPFSKM LFLSESSNWF KKTNGNNSLN SEQEHSPKQL

    VYLMFKKYVK NQSFLSEKNK VTVEQDGFTK NIGLKDMAFP HNMSIFLTTL

    SNVHENGRHN QEKNIQEEIE KEALIEEKVV LPQVHEATGS KNFLKDILIL

    GTRQNISLYE VHVPVLQNIT SINNSTNTVQ IHMEHFFKRR KDKETNSEGL

    VNKTREMVKN YPSQKNITTQ RSKRALGQFR LSTQWLKTIN CSTQCIIKQI

    DHSKEMKKFI TKSSLSDSSV IKSTTQTNSS DSHIVKTSAF PPIDLKRSPF

    QNKFSHVQAS SYIYDFKTKS SRIQESNNFL KETKINNPSL AILPWNMFID

    QGKFTSPGKS NTNSVTYKKR ENIIFLKPTL PEESGKIELL PQVSIQEEEI

    LPTETSHGSP GHLNLMKEVF LQKIQGPTKW NKAKRHGESI KGKTESSKNT

    RSKLLNHHAW DYHYAAQIPK DMWKSKEKSP EIISIKQEDT ILSLRPHGNS

    HSIGANEKQN WPQRETTWVK QGQTQRTCSQ IPPVLKRHQR ELSAFQSEQE

    ATDYDDAITI ETIEDFDIYS EDIKQGPRSF QQKTRHYFIA AVERLWDYGM

    STSHVLRNRY QSDNVPQFKK VVFQEFTDGS FSQPLYRGEL NEHLGLLGPY

    IRAEVEDNIM VTFKNQASRP YSFYSSLISY KEDQRGEEPR RNFVKPNETK

    IYFWKVQHHM APTEDEFDCK AWAYFSDVDL ERDMHSGLIG PLLICHANTL

    NPAHGRQVSV QEFALLFTIF DETKSWYFTE NVKRNCKTPC NFQMEDPTLK

    ENYRFHAING YVMDTLPGLV MAQDQRIRWY LLSMGNNENI QSIHFSGHVF

    TVRKKEEYKM AVYNLYPGVF ETLEMIPSRA GIWRVECLIG EHLQAGMSTL

    FLVYSKQCQI PLGMASGSIR DFQITASGHY GQWAPNLARL HYSGSINAWS

    TKEPFSWIKV DLLAPMIVHG IKTQGARQKF SSLYISQFII MYSLDGKKWL

    SYQGNSTGTL MVFFGNVDSS GIKHNSFNPP IIARYIRLHP THSSIRSTLR

    MELMGCDLNS CSIPLGMESK VISDTQITAS SYFTNMFATW SPSQARLHLQ

    GRTNAWRPQV NDPKQWLQVD LQKTMKVTGI ITQGVKSLFT SMFVKEFLIS

    SSQDGHHWTQ ILYNGKVKVF QGNQDSSTPM MNSLDPPLLT RYLRIHPQIW

    EHQIALRLEI LGCEAQQQY

  • UCSC uc009tpt.3; mouse.;



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