Human MBP ELISA Kit

Alternative name
Human MBP ELISA Kit;Human myelin A1 protein ELISA Kit;Human myelin membrane encephalitogenic protein ELISA Kit;Human myelin basic protein ELISA Kit;Human Golli-MBP ELISA Kit;
Catalog
E002639
species
Human
GeneMBP
Standard Curve
Other Species Human MBP/MBL ELISA Kit Human MBP-Ab ELISA Kit Mouse Mbp ELISA Kit Mouse MBP/MBL ELISA Kit Bovine MBP ELISA Kit Guinea Pig MBP ELISA Kit Chicken MBP ELISA Kit Horse MBP ELISA Kit Porcine MBP ELISA Kit Rabbit MBP ELISA Kit Rat Mbp ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of MBPAb. No significant cross-reactivity or interference between MBPAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MBPAb and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman MBP ELISA Kit allows for the in vitro quantitative determination of MBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: MBPAb ELISA kit applies the competitive enzyme immunoassay technique utilizing myelin basic protein antigen and an MBPAb-HRP conjugate. The assay sample and buffer are incubated together with MBPAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBPAb concentration since MBPAb from samples and MBPAb-HRP conjugate compete for the myelin basic protein antigen binding site. Since the number of sites is limited, as more sites are occupied by MBPAb from the sample, fewer sites are left to bind MBPAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBPAb concentration in each sample is interpolated from this standard curve.
Human Myelin basic protein Protein information
Uniprot ID MBP_HUMAN
Uniprot AC Q6FI04; Q6PK23;
UniGene Hs.551713;
GeneID 4155
KEGG hsa:4155;
Human Myelin basic protein Protein SEQUENCE
SEQUENCE 304 AA; 33117 MW; 4AD7305C1D5434C4 CRC64;
MGNHAGKREL NAEKASTNSE TNRGESEKKR NLGELSRTTS EDNEVFGEAD
ANQNNGTSSQ DTAVTDSKRT ADPKNAWQDA HPADPGSRPH LIRLFSRDAP
GREDNTFKDR PSESDELQTI QEDSAATSES LDVMASQKRP SQRHGSKYLA
TASTMDHARH GFLPRHRDTG ILDSIGRFFG GDRGAPKRGS GKDSHHPART
AHYGSLPQKS HGRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW
GAEGQRPGFG YGGRASDYKS AHKGFKGVDA QGTLSKIFKL GGRDSRSGSP
MARR
UCSC uc002lml.4; human. [P02686-1];

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Human MBP ELISA Kit

Human MBP ELISA Kit;Human myelin A1 protein ELISA Kit;Human myelin membrane encephalitogenic protein ELISA Kit;Human myelin basic protein ELISA Kit;Human Golli-MBP ELISA Kit;