Human MBP ELISA Kit

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  • Alternative name

    Human MBP ELISA Kit;Human myelin A1 protein ELISA Kit;Human myelin membrane encephalitogenic protein ELISA Kit;Human myelin basic protein ELISA Kit;Human Golli-MBP ELISA Kit;

  • Catalog
    E002639
  • species
    Human
  • GeneMBP
  • Standard CurveHuman MBP ELISA Kit
  • Other Species Human MBP/MBL ELISA KitHuman MBP-Ab ELISA KitMouse Mbp ELISA KitMouse MBP/MBL ELISA KitBovine MBP ELISA KitGuinea Pig MBP ELISA KitChicken MBP ELISA KitHorse MBP ELISA KitPorcine MBP ELISA KitRabbit MBP ELISA KitRat Mbp ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MBPAb. No significant cross-reactivity or interference between MBPAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MBPAb and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman MBP ELISA Kit allows for the in vitro quantitative determination of MBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: MBPAb ELISA kit applies the competitive enzyme immunoassay technique utilizing myelin basic protein antigen and an MBPAb-HRP conjugate. The assay sample and buffer are incubated together with MBPAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBPAb concentration since MBPAb from samples and MBPAb-HRP conjugate compete for the myelin basic protein antigen binding site. Since the number of sites is limited, as more sites are occupied by MBPAb from the sample, fewer sites are left to bind MBPAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBPAb concentration in each sample is interpolated from this standard curve.
  • Human Myelin basic protein Protein information
  • Uniprot ID MBP_HUMAN
  • Uniprot AC Q6FI04; Q6PK23;
  • UniGene Hs.551713;
  • GeneID 4155
  • KEGG hsa:4155;
  • Human Myelin basic protein Protein SEQUENCE
  • SEQUENCE 304 AA; 33117 MW; 4AD7305C1D5434C4 CRC64;

    MGNHAGKREL NAEKASTNSE TNRGESEKKR NLGELSRTTS EDNEVFGEAD

    ANQNNGTSSQ DTAVTDSKRT ADPKNAWQDA HPADPGSRPH LIRLFSRDAP

    GREDNTFKDR PSESDELQTI QEDSAATSES LDVMASQKRP SQRHGSKYLA

    TASTMDHARH GFLPRHRDTG ILDSIGRFFG GDRGAPKRGS GKDSHHPART

    AHYGSLPQKS HGRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW

    GAEGQRPGFG YGGRASDYKS AHKGFKGVDA QGTLSKIFKL GGRDSRSGSP

    MARR

  • UCSC uc002lml.4; human. [P02686-1];




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