Human CHI3L2 ELISA Kit

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  • Alternative name

    Human chondrocyte protein 39 ELISA Kit;Human YKL-39 ELISA Kit;Human CHIL2 ELISA Kit;Human YKL39 ELISA Kit;Human chitinase 3 like 2 ELISA Kit;Human chitinase-3-like protein 2 ELISA Kit;

  • Catalog
    E008118
  • species
    Human
  • GeneCHI3L2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Chitinase 3 Like Protein 2 (CHI3L2). No significant cross-reactivity or interference between Chitinase 3 Like Protein 2 (CHI3L2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.133ng/mL.
  • Intended UseHuman CHI3L2 ELISA Kit allows for the in vitro quantitative determination of CHI3L2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase 3 Like Protein 2 (CHI3L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase 3 Like Protein 2 (CHI3L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase 3 Like Protein 2 (CHI3L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chitinase 3 Like Protein 2 (CHI3L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Chitinase-3-like protein 2 Protein information
  • Uniprot ID CH3L2_HUMAN
  • Uniprot AC Q15782; A6NNY3; B4DPR7; Q15749; Q15783; Q5VUV7; Q96F97;
  • UniGene Hs.514840;
  • GeneID 1117
  • KEGG hsa:1117;
  • Human Chitinase-3-like protein 2 Protein SEQUENCE
  • SEQUENCE 390 AA; 43501 MW; 97B86A2F3AA35677 CRC64;

    MGATTMDQKS LWAGVVVLLL LQGGSAYKLV CYFTNWSQDR QEPGKFTPEN

    IDPFLCSHLI YSFASIENNK VIIKDKSEVM LYQTINSLKT KNPKLKILLS

    IGGYLFGSKG FHPMVDSSTS RLEFINSIIL FLRNHNFDGL DVSWIYPDQK

    ENTHFTVLIH ELAEAFQKDF TKSTKERLLL TAGVSAGRQM IDNSYQVEKL

    AKDLDFINLL SFDFHGSWEK PLITGHNSPL SKGWQDRGPS SYYNVEYAVG

    YWIHKGMPSE KVVMGIPTYG HSFTLASAET TVGAPASGPG AAGPITESSG

    FLAYYEICQF LKGAKITRLQ DQQVPYAVKG NQWVGYDDVK SMETKVQFLK

    NLNLGGAMIW SIDMDDFTGK SCNQGPYPLV QAVKRSLGSL

  • UCSC uc001eam.4; human. [Q15782-4];



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