Human NF2 ELISA Kit

Alternative name
Human moesin-ezrin-radixin-like protein ELISA Kit;Human Neurofibromin-2 ELISA Kit;Human schwannomerlin ELISA Kit;Human schwannomin ELISA Kit;Human SCH ELISA Kit;Human ACN ELISA Kit;Human BANF ELISA Kit;Human neurofibromin 2 ELISA Kit;Human merlin ELISA Kit;Human moesin-ezrin-radixin like ELISA Kit;Human moesin-ezrin-radizin-like protein ELISA Kit;Human neurofibromin 2 (bilateral acoustic neuroma) ELISA Kit;
Catalog
E026149
species
Human
GeneNF2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurofibromin 2 (NF2). No significant cross-reactivity or interference between Neurofibromin 2 (NF2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.117ng/mL.
Intended UseHuman NF2 ELISA Kit allows for the in vitro quantitative determination of NF2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofibromin 2 (NF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofibromin 2 (NF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofibromin 2 (NF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofibromin 2 (NF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Merlin Protein information
Uniprot ID MERL_HUMAN
Uniprot AC Q96T33; Q9BTW3; Q9UNG9; Q9UNH3; Q9UNH4;
UniGene Hs.187898;
GeneID 4771
KEGG hsa:4771;
Human Merlin Protein SEQUENCE
SEQUENCE 595 AA; 69690 MW; B1A1BF2BD5DA561C CRC64;
MAGAIASRMS FSSLKRKQPK TFTVRIVTMD AEMEFNCEMK WKGKDLFDLV
CRTLGLRETW FFGLQYTIKD TVAWLKMDKK VLDHDVSKEE PVTFHFLAKF
YPENAEEELV QEITQHLFFL QVKKQILDEK IYCPPEASVL LASYAVQAKY
GDYDPSVHKR GFLAQEELLP KRVINLYQMT PEMWEERITA WYAEHRGRAR
DEAEMEYLKI AQDLEMYGVN YFAIRNKKGT ELLLGVDALG LHIYDPENRL
TPKISFPWNE IRNISYSDKE FTIKPLDKKI DVFKFNSSKL RVNKLILQLC
IGNHDLFMRR RKADSLEVQQ MKAQAREEKA RKQMERQRLA REKQMREEAE
RTRDELERRL LQMKEEATMA NEALMRSEET ADLLAEKAQI TEEEAKLLAQ
KAAEAEQEMQ RIKATAIRTE EEKRLMEQKV LEAEVLALKM AEESERRAKE
ADQLKQDLQE AREAERRAKQ KLLEIATKPT YPPMNPIPAP LPPDIPSFNL
IGDSLSFDFK DTDMKRLSME IEKEKVEYME KSKHLQEQLN ELKTEIEALK
LKERETALDI LHNENSDRGG SSKHNTIKKL TLQSAKSRVA FFEEL
UCSC uc003afy.5; human. [P35240-1];

Category

Human NF2 ELISA Kit