Human MUC4 ELISA Kit

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  • Alternative name

    Human MUC-4 ELISA Kit;Human ascites sialoglycoprotein ELISA Kit;Human ASGP ELISA Kit;Human pancreatic adenocarcinoma mucin ELISA Kit;Human testis mucin ELISA Kit;Human tracheobronchial mucin ELISA Kit;Human Ascites sialoglycoprotein 1 ELISA Kit;Human ASGP-1 ELISA Kit;Human Ascites sialoglycoprotein 2 ELISA Kit;Human ASGP-2 ELISA Kit;Human HSA276359 ELISA Kit;Human mucin 4, cell surface associated ELISA Kit;Human mucin-4 ELISA Kit;Human mucin 4, tracheobronchial ELISA Kit;

  • Catalog
    E025046
  • species
    Human
  • GeneMUC4
  • Standard CurveHuman MUC4 ELISA Kit
  • Other Species Mouse Muc4 ELISA KitRat Muc4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MUC4 ELISA Kit allows for the in vitro quantitative determination of MUC4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This MUC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MUC4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MUC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MUC4 antibody and an MUC4-HRP conjugate. The assay sample and buffer are incubated together with MUC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC4 concentration since MUC4 from samples and MUC4-HRP conjugate compete for the anti-MUC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC4 from the sample, fewer sites are left to bind MUC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC4 concentration in each sample is interpolated from this standard curve.
  • Human Mucin-4 beta chain Protein information
  • Uniprot ID MUC4_HUMAN
  • Uniprot AC Q9NY76; Q9NY77; Q9NY78; Q9NY79; Q9NY80; Q9NY81;
  • UniGene Hs.369646;
  • GeneID 4585
  • KEGG hsa:4585;
  • Human Mucin-4 beta chain Protein SEQUENCE
  • SEQUENCE 2169 AA; 231518 MW; 53615AF8DD416957 CRC64;

    MKGARWRRVP WVSLSCLCLC LLPHVVPGTT EDTLITGSKT AAPVTSTGST

    TATLEGQSTA ASSRTSNQDI SASSQNHQTK STETTSKAQT DTLTQMMTST

    LFSSPSVHNV METVTQETAP PDEMTTSFPS SVTNTLMMTS KTITMTTSTD

    STLGNTEETS TAGTESSTPV TSAVSITAGQ EGQSRTTSWR TSIQDTSASS

    QNHWTRSTQT TRESQTSTLT HRTTSTPSFS PSVHNVTGTV SQKTSPSGET

    ATSSLCSVTN TSMMTSEKIT VTTSTGSTLG NPGETSSVPV TGSLMPVTSA

    ALVTVDPEGQ SPATFSRTST QDTTAFSKNH QTQSVETTRV SQINTLNTLT

    PVTTSTVLSS PSGFNPSGTV SQETFPSGET TISSPSSVSN TFLVTSKVFR

    MPISRDSTLG NTEETSLSVS GTISAITSKV STIWWSDTLS TALSPSSLPP

    KISTAFHTQQ SEGAETTGRP HERSSFSPGV SQEIFTLHET TTWPSSFSSK

    GHTTWSQTEL PSTSTGAATR LVTGNPSTRA AGTIPRVPSK VSAIGEPGEP

    TTYSSHSTTL PKTTGAGAQT QWTQETGTTG EALLSSPSYS VIQMIKTATS

    PSSSPMLDRH TSQQITTAPS TNHSTIHSTS TSPQESPAVS QRGHTRAPQT

    TQESQTTRSV SPMTDTKTVT TPGSSFTASG HSPSEIVPQD APTISAATTF

    APAPTGNGHT TQAPTTALQA APSSHDATLG PSGGTSLSKT GALTLANSVV

    STPGGPEGQW TSASASTSPD TAAAMTHTHQ AESTEASGQT QTSEPASSGS

    RTTSAGTATP SSSGASGTTP SGSEGISTSG ETTRFSSNPS RDSHTTQSTT

    ELLSASASHG AIPVSTGMAS SIVPGTFHPT LSEASTAGRP TGQSSPTSPS

    ASPQETAAIS RMAQTQRTGT SRGSDTISLA SQATDTFSTV PPTPPSITSS

    GLTSPQTQTH TLSPSGSGKT FTTALISNAT PLPVTSTSSA STGHATPLAV

    SSATSASTVS SDSPLKMETS GMTTPSLKTD GGRRTATSPP PTTSQTIIST

    IPSTAMHTRS TAAPIPILPE RGVSLFPYGA GAGDLEFVRR TVDFTSPLFK

    PATGFPLGSS LRDSLYFTDN GQIIFPESDY QIFSYPNPLP TGFTGRDPVA

    LVAPFWDDAD FSTGRGTTFY QEYETFYGEH SLLVQQAESW IRKMTNNGGY

    KARWALKVTW VNAHAYPAQW TLGSNTYQAI LSTDGSRSYA LFLYQSGGMQ

    WDVAQRSGNP VLMGFSSGDG YFENSPLMSQ PVWERYRPDR FLNSNSGLQG

    LQFYRLHREE RPNYRLECLQ WLKSQPRWPS WGWNQVSCPC SWQQGRRDLR

    FQPVSIGRWG LGSRQLCSFT SWRGGVCCSY GPWGEFREGW HVQRPWQLAQ

    ELEPQSWCCR WNDKPYLCAL YQQRRPHVGC ATYRPPQPAW MFGDPHITTL

    DGVSYTFNGL GDFLLVGAQD GNSSFLLQGR TAQTGSAQAT NFIAFAAQYR

    SSSLGPVTVQ WLLEPHDAIR VLLDNQTVTF QPDHEDGGGQ ETFNATGVLL

    SRNGSEVSAS FDGWATVSVI ALSNILHASA SLPPEYQNRT EGLLGVWNNN

    PEDDFRMPNG STIPPGSPEE MLFHFGMTWQ INGTGLLGKR NDQLPSNFTP

    VFYSQLQKNS SWAEHLISNC DGDSSCIYDT LALRNASIGL HTREVSKNYE

    QANATLNQYP PSINGGRVIE AYKGQTTLIQ YTSNAEDANF TLRDSCTDLE

    LFENGTLLWT PKSLEPFTLE ILARSAKIGL ASALQPRTVV CHCNAESQCL

    YNQTSRVGNS SLEVAGCKCD GGTFGRYCEG SEDACEEPCF PSVHCVPGKG

    CEACPPNLTG DGRHCAALGS SFLCQNQSCP VNYCYNQGHC YISQTLGCQP

    MCTCPPAFTD SRCFLAGNNF SPTVNLELPL RVIQLLLSEE ENASMAEVNA

    SVAYRLGTLD MRAFLRNSQV ERIDSAAPAS GSPIQHWMVI SEFQYRPRGP

    VIDFLNNQLL AAVVEAFLYH VPRRSEEPRN DVVFQPISGE DVRDVTALNV

    STLKAYFRCD GYKGYDLVYS PQSGFTCVSP CSRGYCDHGG QCQHLPSGPR

    CSCVSFSIYT AWGEHCEHLS MKLDAFFGIF FGALGGLLLL GVGTFVVLRF

    WGCSGARFSY FLNSAEALP

  • UCSC uc003fvo.5; human. [Q99102-1];


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