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Alternative name
Human pro-NRG4 ELISA Kit;Human NRG-4 ELISA Kit;Human HRG4 ELISA Kit;Human neuregulin 4 ELISA Kit;Human pro-neuregulin-4, membrane-bound isoform ELISA Kit;Human heregulin 4 ELISA Kit;
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Catalog
E026073
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species
Human
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GeneNRG4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of NRG4. No significant cross-reactivity or interference between NRG4 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of NRG4 is typically less than 0.065ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
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Intended UseHuman NRG4 ELISA Kit allows for the in vitro quantitative determination of NRG4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NRG4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NRG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NRG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NRG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Neuregulin-4 Protein information
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Uniprot ID
NRG4_HUMAN
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Uniprot AC
Q8WWG1; A6NIE8;
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UniGene
Hs.732438;
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GeneID
145957
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KEGG
hsa:145957;
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Human Neuregulin-4 Protein SEQUENCE
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SEQUENCE 115 AA; 12722 MW; 72F962E2D0F37AC3 CRC64;
MPTDHEEPCG PSHKSFCLNG GLCYVIPTIP SPFCRCVENY TGARCEEVFL
PGSSIQTKSN
LFEAFVALAV LVTLIIGAFY FLCRKGHFQR ASSVQYDINL
VETSSTSAHH SHEQH
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UCSC
uc002bbo.5; human.;
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