Human Nephrin ELISA Kit

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  • Alternative name

    Nephrin ELISA KIT; Renal glomerulus-specific cell adhesion receptorNPHS1 ELISA KIT; NPHN ELISA KIT

  • Catalog
    E025949
  • species
    Human
  • GeneNephrin
  • Other Species Mouse Nephrin ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NPHN. No significant cross-reactivity or interference between NPHN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPHN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman Nephrin ELISA Kit allows for the in vitro quantitative determination of Nephrin , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This NPHN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NPHN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||NPHN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NPHN. Standards or samples are then added to the microtiter plate wells and NPHN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NPHN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for NPHN are added to each well to "sandwich" the NPHN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NPHN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPHN concentration in each sample is interpolated from this standard curve.




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