Human DAPK1 ELISA Kit

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  • Alternative name

    Human DAP kinase 1 ELISA Kit;Human DAPK ELISA Kit;Human death associated protein kinase 1 ELISA Kit;Human death-associated protein kinase 1 ELISA Kit;

  • Catalog
    E011436
  • species
    Human
  • GeneDAPK1
  • Standard CurveHuman DAPK1 ELISA Kit
  • Other Species Mouse Dapk1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Death Associated Protein Kinase 1 (DAPK1). No significant cross-reactivity or interference between Death Associated Protein Kinase 1 (DAPK1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseHuman DAPK1 ELISA Kit allows for the in vitro quantitative determination of DAPK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death Associated Protein Kinase 1 (DAPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death Associated Protein Kinase 1 (DAPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death Associated Protein Kinase 1 (DAPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Death Associated Protein Kinase 1 (DAPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Death-associated protein kinase 1 Protein information
  • Uniprot ID DAPK1_HUMAN
  • Uniprot AC P53355; B7ZLD2; B7ZLE7; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8;
  • UniGene Hs.380277; Hs.693441;
  • GeneID 1612
  • KEGG hsa:1612;
  • Human Death-associated protein kinase 1 Protein SEQUENCE
  • SEQUENCE 1430 AA; 160046 MW; E2C4246E7C78A6D2 CRC64;

    MTVFRQENVD DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK

    SSRRGVSRED IEREVSILKE IQHPNVITLH EVYENKTDVI LILELVAGGE

    LFDFLAEKES LTEEEATEFL KQILNGVYYL HSLQIAHFDL KPENIMLLDR

    NVPKPRIKII DFGLAHKIDF GNEFKNIFGT PEFVAPEIVN YEPLGLEADM

    WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYEFEDEY FSNTSALAKD

    FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKFKKFA

    ARKKWKQSVR LISLCQRLSR SFLSRSNMSV ARSDDTLDEE DSFVMKAIIH

    AINDDNVPGL QHLLGSLSNY DVNQPNKHGT PPLLIAAGCG NIQILQLLIK

    RGSRIDVQDK GGSNAVYWAA RHGHVDTLKF LSENKCPLDV KDKSGEMALH

    VAARYGHADV AQLLCSFGSN PNIQDKEEET PLHCAAWHGY YSVAKALCEA

    GCNVNIKNRE GETPLLTASA RGYHDIVECL AEHGADLNAC DKDGHIALHL

    AVRRCQMEVI KTLLSQGCFV DYQDRHGNTP LHVACKDGNM PIVVALCEAN

    CNLDISNKYG RTPLHLAANN GILDVVRYLC LMGASVEALT TDGKTAEDLA

    RSEQHEHVAG LLARLRKDTH RGLFIQQLRP TQNLQPRIKL KLFGHSGSGK

    TTLVESLKCG LLRSFFRRRR PRLSSTNSSR FPPSPLASKP TVSVSINNLY

    PGCENVSVRS RSMMFEPGLT KGMLEVFVAP THHPHCSADD QSTKAIDIQN

    AYLNGVGDFS VWEFSGNPVY FCCYDYFAAN DPTSIHVVVF SLEEPYEIQL

    NQVIFWLSFL KSLVPVEEPI AFGGKLKNPL QVVLVATHAD IMNVPRPAGG

    EFGYDKDTSL LKEIRNRFGN DLHISNKLFV LDAGASGSKD MKVLRNHLQE

    IRSQIVSVCP PMTHLCEKII STLPSWRKLN GPNQLMSLQQ FVYDVQDQLN

    PLASEEDLRR IAQQLHSTGE INIMQSETVQ DVLLLDPRWL CTNVLGKLLS

    VETPRALHHY RGRYTVEDIQ RLVPDSDVEE LLQILDAMDI CARDLSSGTM

    VDVPALIKTD NLHRSWADEE DEVMVYGGVR IVPVEHLTPF PCGIFHKVQV

    NLCRWIHQQS TEGDADIRLW VNGCKLANRG AELLVLLVNH GQGIEVQVRG

    LETEKIKCCL LLDSVCSTIE NVMATTLPGL LTVKHYLSPQ QLREHHEPVM

    IYQPRDFFRA QTLKETSLTN TMGGYKESFS SIMCFGCHDV YSQASLGMDI

    HASDLNLLTR RKLSRLLDPP DPLGKDWCLL AMNLGLPDLV AKYNTSNGAP

    KDFLPSPLHA LLREWTTYPE STVGTLMSKL RELGRRDAAD FLLKASSVFK

    INLDGNGQEA YASSCNSGTS YNSISSVVSR

  • UCSC uc004apc.5; human. [P53355-1];


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