Human NAAA ELISA Kit

Alternative name
Human acid ceramidase-like protein ELISA Kit;Human N-acylsphingosine amidohydrolase-like ELISA Kit;Human ASAH-like protein ELISA Kit;Human ASAHL ELISA Kit;Human PLT ELISA Kit;Human N-acylethanolamine acid amidase ELISA Kit;Human N-acylethanolamine-hydrolyzing acid amidase ELISA Kit;
Catalog
E025644
species
Human
GeneNAAA
Standard Curve
Other Species Mouse Naaa ELISA Kit Rat Naaa ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Acylethanolamine Acid Amidase (NAAA). No significant cross-reactivity or interference between N-Acylethanolamine Acid Amidase (NAAA) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.124ng/mL.
Intended UseHuman NAAA ELISA Kit allows for the in vitro quantitative determination of NAAA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylethanolamine Acid Amidase (NAAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acylethanolamine Acid Amidase (NAAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylethanolamine Acid Amidase (NAAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylethanolamine Acid Amidase (NAAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human N-acylethanolamine-hydrolyzing acid amidase subunit beta Protein information
Uniprot ID NAAA_HUMAN
Uniprot AC Q02083; Q5KTF2; Q96EY2; Q9BRA8;
UniGene Hs.437365;
GeneID 27163
KEGG hsa:27163;
Human N-acylethanolamine-hydrolyzing acid amidase subunit beta Protein SEQUENCE
SEQUENCE 359 AA; 40066 MW; A8E214DB383B872A CRC64;
MRTADREARP GLPSLLLLLL AGAGLSAASP PAAPRFNVSL DSVPELRWLP
VLRHYDLDLV RAAMAQVIGD RVPKWVHVLI GKVVLELERF LPQPFTGEIR
GMCDFMNLSL ADCLLVNLAY ESSVFCTSIV AQDSRGHIYH GRNLDYPFGN
VLRKLTVDVQ FLKNGQIAFT GTTFIGYVGL WTGQSPHKFT VSGDERDKGW
WWENAIAALF RRHIPVSWLI RATLSESENF EAAVGKLAKT PLIADVYYIV
GGTSPREGVV ITRNRDGPAD IWPLDPLNGA WFRVETNYDH WKPAPKEDDR
RTSAIKALNA TGQANLSLEA LFQILSVVPV YNNFTIYTTV MSAGSPDKYM
TRIRNPSRK
UCSC uc003hjb.4; human. [Q02083-1];

Category

Human NAAA ELISA Kit

Human acid ceramidase-like protein ELISA Kit;Human N-acylsphingosine amidohydrolase-like ELISA Kit;Human ASAH-like protein ELISA Kit;Human ASAHL ELISA Kit;Human PLT ELISA Kit;Human N-acylethanolamine acid amidase ELISA Kit;Human N-acylethanolamine-hydrolyzing acid amidase ELISA Kit;