Human NAAA ELISA Kit

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  • Alternative name

    Human acid ceramidase-like protein ELISA Kit;Human N-acylsphingosine amidohydrolase-like ELISA Kit;Human ASAH-like protein ELISA Kit;Human ASAHL ELISA Kit;Human PLT ELISA Kit;Human N-acylethanolamine acid amidase ELISA Kit;Human N-acylethanolamine-hydrolyzing acid amidase ELISA Kit;

  • Catalog
    E025644
  • species
    Human
  • GeneNAAA
  • Standard CurveHuman NAAA ELISA Kit
  • Other Species Mouse Naaa ELISA KitRat Naaa ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Acylethanolamine Acid Amidase (NAAA). No significant cross-reactivity or interference between N-Acylethanolamine Acid Amidase (NAAA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.124ng/mL.
  • Intended UseHuman NAAA ELISA Kit allows for the in vitro quantitative determination of NAAA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylethanolamine Acid Amidase (NAAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acylethanolamine Acid Amidase (NAAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylethanolamine Acid Amidase (NAAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylethanolamine Acid Amidase (NAAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human N-acylethanolamine-hydrolyzing acid amidase subunit beta Protein information
  • Uniprot ID NAAA_HUMAN
  • Uniprot AC Q02083; Q5KTF2; Q96EY2; Q9BRA8;
  • UniGene Hs.437365;
  • GeneID 27163
  • KEGG hsa:27163;
  • Human N-acylethanolamine-hydrolyzing acid amidase subunit beta Protein SEQUENCE
  • SEQUENCE 359 AA; 40066 MW; A8E214DB383B872A CRC64;

    MRTADREARP GLPSLLLLLL AGAGLSAASP PAAPRFNVSL DSVPELRWLP

    VLRHYDLDLV RAAMAQVIGD RVPKWVHVLI GKVVLELERF LPQPFTGEIR

    GMCDFMNLSL ADCLLVNLAY ESSVFCTSIV AQDSRGHIYH GRNLDYPFGN

    VLRKLTVDVQ FLKNGQIAFT GTTFIGYVGL WTGQSPHKFT VSGDERDKGW

    WWENAIAALF RRHIPVSWLI RATLSESENF EAAVGKLAKT PLIADVYYIV

    GGTSPREGVV ITRNRDGPAD IWPLDPLNGA WFRVETNYDH WKPAPKEDDR

    RTSAIKALNA TGQANLSLEA LFQILSVVPV YNNFTIYTTV MSAGSPDKYM

    TRIRNPSRK

  • UCSC uc003hjb.4; human. [Q02083-1];



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