Human IRF3 ELISA Kit

Alternative name
Human IRF-3 ELISA Kit;Human IIAE7 ELISA Kit;Human interferon regulatory factor 3 ELISA Kit;
Catalog
E020332
species
Human
GeneIRF3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Regulatory Factor 3 (IRF3). No significant cross-reactivity or interference between Interferon Regulatory Factor 3 (IRF3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.065ng/mL.
Intended UseHuman IRF3 ELISA Kit allows for the in vitro quantitative determination of IRF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 3 (IRF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 3 (IRF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 3 (IRF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Regulatory Factor 3 (IRF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Interferon regulatory factor 3 Protein information
Uniprot ID IRF3_HUMAN
Uniprot AC Q14653; A8K7L2; B2RAZ3; Q5FBY1; Q5FBY2; Q5FBY4; Q7Z5G6;
UniGene Hs.289052; Hs.731922; Hs.75254;
GeneID 3661
KEGG hsa:3661;
Human Interferon regulatory factor 3 Protein SEQUENCE
SEQUENCE 427 AA; 47219 MW; F536676FA78B0110 CRC64;
MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED
FGIFQAWAEA TGAYVPGRDK PDLPTWKRNF RSALNRKEGL RLAEDRSKDP
HDPHKIYEFV NSGVGDFSQP DTSPDTNGGG STSDTQEDIL DELLGNMVLA
PLPDPGPPSL AVAPEPCPQP LRSPSLDNPT PFPNLGPSEN PLKRLLVPGE
EWEFEVTAFY RGRQVFQQTI SCPEGLRLVG SEVGDRTLPG WPVTLPDPGM
SLTDRGVMSY VRHVLSCLGG GLALWRAGQW LWAQRLGHCH TYWAVSEELL
PNSGHGPDGE VPKDKEGGVF DLGPFIVDLI TFTEGSGRSP RYALWFCVGE
SWPQDQPWTK RLVMVKVVPT CLRALVEMAR VGGASSLENT VDLHISNSHP
LSLTSDQYKA YLQDLVEGMD FQGPGES
UCSC uc002pot.3; human. [Q14653-1];

Category

Human IRF3 ELISA Kit

Human IRF-3 ELISA Kit;Human IIAE7 ELISA Kit;Human interferon regulatory factor 3 ELISA Kit;