Human AMPH ELISA Kit

Alternative name
Human AMPH1 ELISA Kit;Human amphiphysin ELISA Kit;Human amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) ELISA Kit;Human amphiphysin I ELISA Kit;
Catalog
E002511
species
Human
GeneAMPH
Standard Curve
Other Species Mouse Amph ELISA Kit Chicken AMPH ELISA Kit Rat Amph ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Anti-AMPH. No significant cross-reactivity or interference between Anti-AMPH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-AMPH and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman AMPH ELISA Kit allows for the in vitro quantitative determination of AMPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: Anti-AMPH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with AMPH antigen. Standards or samples are then added to the microtiter plate wells and anti-AMPH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of anti-AMPH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated AMPH antigen is added to each well to "sandwich" the Anti-AMPH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-AMPH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-AMPH concentration in each sample is interpolated from this standard curve.
Human Amphiphysin Protein information
Uniprot ID AMPH_HUMAN
Uniprot AC P49418; A4D1X8; A4D1X9; O43538; Q75MJ8; Q75MK5; Q75MM3; Q8N4G0;
UniGene Hs.592182;
GeneID 273
KEGG hsa:273;
Human Amphiphysin Protein SEQUENCE
SEQUENCE 695 AA; 76257 MW; 78B4F75AB75BA357 CRC64;
MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE
AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE
KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH
HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG
FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFTIQGAPS
DSGPLRIAKT PSPPEEPSPL PSPTASPNHT LAPASPAPAR PRSPSQTRKG
PPVPPLPKVT PTKELQQENI ISFFEDNFVP EISVTTPSQN EVPEVKKEET
LLDLDFDPFK PEVTPAGSAG VTHSPMSQTL PWDLWTTSTD LVQPASGGSF
NGFTQPQDTS LFTMQTDQSM ICNLAESEQA PPTEPKAEEP LAAVTPAVGL
DLGMDTRAEE PVEEAVIIPG ADADAAVGTL VSAAEGAPGE EAEAEKATVP
AGEGVSLEEA KIGTETTEGA ESAQPEAEEL EATVPQEKVI PSVVIEPASN
HEEEGENEIT IGAEPKETTE DAAPPGPTSE TPELATEQKP IQDPQPTPSA
PAMGAADQLA SAREASQELP PGFLYKVETL HDFEAANSDE LTLQRGDVVL
VVPSDSEADQ DAGWLVGVKE SDWLQYRDLA TYKGLFPENF TRRLD
UCSC uc003tgu.4; human. [P49418-1];

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anti-Amphiphysin antibody

Category

Human AMPH ELISA Kit

Human AMPH1 ELISA Kit;Human amphiphysin ELISA Kit;Human amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) ELISA Kit;Human amphiphysin I ELISA Kit;