Human MIP-1 alpha ELISA Kit

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  • Alternative name

    C-C motif chemokine ELISA KIT; LOC100302540 ELISA KIT;

  • Catalog
  • species
  • GeneMIP-1 alpha
  • Standard CurveHuman MIP-1 alpha ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman MIP-1 alpha ELISA Kit allows for the in vitro quantitative determination of MIP-1 alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCytokines, Growth Factors & Hormones; Elisa Kits; Metabolism Assays; Other Metabolism-Related Assays; Metabolism Assays; Metabolism Assays (A-Z); Stem Cell Research Tools; Assay Kits
  • Product Description
    Product Information: MIP-1 Alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human MIP-1 alpha in serum, plasma, urine & cell culture supernatants. Detection Range: 6 pg/ml - 6000 pg/ml. 100 assays. Background: Biobool's Human MIP-1 alpha (Macrophage Inflammatory Protein-1 alpha) or chemokine ligand 3 (CCL3) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-1 alpha. This assay employs an antibody specific for human MIP-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIP-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of MIP-1 alpha is typically less than 6 pg/ml. Detection Range: 6 pg/ml - 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
    Summary: Detection method- Absorbance (450 nm) Species reactivity- human Application- Quantitative protein detection, establishing normal range, validation of antibody array results

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