Human ANXA13 ELISA Kit

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  • Alternative name

    Human annexin XIII ELISA Kit;Human annexin-13 ELISA Kit;Human intestine-specific annexin ELISA Kit;Human ISA ELISA Kit;Human ANX13 ELISA Kit;Human annexin A13 ELISA Kit;Human annexin, intestine-specific ELISA Kit;

  • Catalog
    E002399
  • species
    Human
  • GeneANXA13
  • Standard CurveHuman ANXA13 ELISA Kit
  • Other Species Mouse Anxa13 ELISA KitCanine ANXA13 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ANX-A13. No significant cross-reactivity or interference between ANX-A13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANX-A13 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman ANXA13 ELISA Kit allows for the in vitro quantitative determination of ANXA13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: ANX-A13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANX-A13 antibody and an ANX-A13-HRP conjugate. The assay sample and buffer are incubated together with ANX-A13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANX-A13 concentration since ANX-A13 from samples and ANX-A13-HRP conjugate compete for the anti-ANX-A13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANX-A13 from the sample, fewer sites are left to bind ANX-A13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANX-A13 concentration in each sample is interpolated from this standard curve.
  • Human Annexin A13 Protein information
  • Uniprot ID ANX13_HUMAN
  • Uniprot AC P27216; Q9BQR5;
  • UniGene Hs.181107;
  • GeneID 312
  • KEGG hsa:312;
  • Human Annexin A13 Protein SEQUENCE
  • SEQUENCE 316 AA; 35415 MW; 3F61A1AECE5A067D CRC64;

    MGNRHAKASS PQGFDVDRDA KKLNKACKGM GTNEAAIIEI LSGRTSDERQ

    QIKQKYKATY GKELEEVLKS ELSGNFEKTA LALLDRPSEY AARQLQKAMK

    GLGTDESVLI EVLCTRTNKE IIAIKEAYQR LFDRSLESDV KGDTSGNLKK

    ILVSLLQANR NEGDDVDKDL AGQDAKDLYD AGEGRWGTDE LAFNEVLAKR

    SYKQLRATFQ AYQILIGKDI EEAIEEETSG DLQKAYLTLV RCAQDCEDYF

    AERLYKSMKG AGTDEETLIR IVVTRAEVDL QGIKAKFQEK YQKSLSDMVR

    SDTSGDFRKL LVALLH

  • UCSC uc003yqt.4; human. [P27216-1];


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