Human ANXA13 ELISA Kit

Alternative name
Human annexin XIII ELISA Kit;Human annexin-13 ELISA Kit;Human intestine-specific annexin ELISA Kit;Human ISA ELISA Kit;Human ANX13 ELISA Kit;Human annexin A13 ELISA Kit;Human annexin, intestine-specific ELISA Kit;
Catalog
E002399
species
Human
GeneANXA13
Standard Curve
Other Species Mouse Anxa13 ELISA Kit Canine ANXA13 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ANX-A13. No significant cross-reactivity or interference between ANX-A13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANX-A13 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman ANXA13 ELISA Kit allows for the in vitro quantitative determination of ANXA13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: ANX-A13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANX-A13 antibody and an ANX-A13-HRP conjugate. The assay sample and buffer are incubated together with ANX-A13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANX-A13 concentration since ANX-A13 from samples and ANX-A13-HRP conjugate compete for the anti-ANX-A13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANX-A13 from the sample, fewer sites are left to bind ANX-A13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANX-A13 concentration in each sample is interpolated from this standard curve.
Human Annexin A13 Protein information
Uniprot ID ANX13_HUMAN
Uniprot AC P27216; Q9BQR5;
UniGene Hs.181107;
GeneID 312
KEGG hsa:312;
Human Annexin A13 Protein SEQUENCE
SEQUENCE 316 AA; 35415 MW; 3F61A1AECE5A067D CRC64;
MGNRHAKASS PQGFDVDRDA KKLNKACKGM GTNEAAIIEI LSGRTSDERQ
QIKQKYKATY GKELEEVLKS ELSGNFEKTA LALLDRPSEY AARQLQKAMK
GLGTDESVLI EVLCTRTNKE IIAIKEAYQR LFDRSLESDV KGDTSGNLKK
ILVSLLQANR NEGDDVDKDL AGQDAKDLYD AGEGRWGTDE LAFNEVLAKR
SYKQLRATFQ AYQILIGKDI EEAIEEETSG DLQKAYLTLV RCAQDCEDYF
AERLYKSMKG AGTDEETLIR IVVTRAEVDL QGIKAKFQEK YQKSLSDMVR
SDTSGDFRKL LVALLH
UCSC uc003yqt.4; human. [P27216-1];

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Category

Human ANXA13 ELISA Kit

Human annexin XIII ELISA Kit;Human annexin-13 ELISA Kit;Human intestine-specific annexin ELISA Kit;Human ISA ELISA Kit;Human ANX13 ELISA Kit;Human annexin A13 ELISA Kit;Human annexin, intestine-specific ELISA Kit;