Mouse AGC ELISA Kit

Contact Us
  • Alternative name

    Aggrecan core protein ELISA KIT; Cartilage-specific proteoglycan core protein ELISA KIT; CSPCPAcan ELISA KIT; Agc ELISA KIT; Agc1 ELISA KIT; CSPCP ELISA KIT

  • Catalog
    E043090
  • species
    Mouse
  • GeneAGC
  • Standard CurveMouse AGC ELISA Kit
  • Other Species Human AGC ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AGC. No significant cross-reactivity or interference between AGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGC and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0ng/mL.
  • Intended UseMouse AGC ELISA Kit allows for the in vitro quantitative determination of AGC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses||This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.


Related products with Mouse AGC ELISA Kit

Inquiry About Mouse AGC ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!