Human MC5R ELISA Kit

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  • Alternative name

    Human MC5-R ELISA Kit;Human MC-2 ELISA Kit;Human MC2 ELISA Kit;Human melanocortin 5 receptor ELISA Kit;Human melanocortin receptor 5 ELISA Kit;

  • Catalog
    E023980
  • species
    Human
  • GeneMC5R
  • Standard CurveHuman MC5R ELISA Kit
  • Other Species Mouse Mc5r ELISA KitBovine MC5R ELISA KitPorcine MC5R ELISA KitRat Mc5r ELISA KitSheep MC5R ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MC5R. No significant cross-reactivity or interference between MC5R and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MC5R is typically less than 0.25ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseHuman MC5R ELISA Kit allows for the in vitro quantitative determination of MC5R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MC5R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MC5R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MC5R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MC5R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Melanocortin receptor 5 Protein information
  • Uniprot ID MC5R_HUMAN
  • Uniprot AC P33032; B0YJ34; Q502V1;
  • UniGene Hs.248145;
  • GeneID 4161
  • KEGG hsa:4161;
  • Human Melanocortin receptor 5 Protein SEQUENCE
  • SEQUENCE 325 AA; 36601 MW; 8BEC17E1BDA059BB CRC64;

    MNSSFHLHFL DLNLNATEGN LSGPNVKNKS SPCEDMGIAV EVFLTLGVIS

    LLENILVIGA IVKNKNLHSP MYFFVCSLAV ADMLVSMSSA WETITIYLLN

    NKHLVIADAF VRHIDNVFDS MICISVVASM CSLLAIAVDR YVTIFYALRY

    HHIMTARRSG AIIAGIWAFC TGCGIVFILY SESTYVILCL ISMFFAMLFL

    LVSLYIHMFL LARTHVKRIA ALPGASSARQ RTSMQGAVTV TMLLGVFTVC

    WAPFFLHLTL MLSCPQNLYC SRFMSHFNMY LILIMCNSVM DPLIYAFRSQ

    EMRKTFKEII CCRGFRIACS FPRRD

  • UCSC uc010xaf.3; human.;


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