SpecificityThis assay has high sensitivity and excellent specificity for detection of MMP4. No significant cross-reactivity or interference between MMP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP4 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman MMP4 ELISA Kit allows for the in vitro quantitative determination of MMP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyHuman ELISA Kit
Product Description specificalPrinciple of the Assay: MMP4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP4. Standards or samples are then added to the microtiter plate wells and MMP4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for MMP4 are added to each well to "sandwich" the MMP4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP4 concentration in each sample is interpolated from this standard curve.