Human MCT ELISA Kit

Contact Us
  • Alternative name

    Monocarboxylate transporter 6 ELISA KIT; Monocarboxylate transporter 5 ELISA KIT; MCT 5 ELISA KIT; Solute carrier family 16 member 5SLC16A5 ELISA KIT; MCT5 ELISA KIT; MCT6 ELISA KIT; MCT 6 ELISA KIT; MCT 5 ELISA KIT

  • Catalog
    E023692
  • species
    Human
  • GeneMCT
  • Standard CurveHuman MCT ELISA Kit
  • Other Species Mouse MCT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MCH. No significant cross-reactivity or interference between MCH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MCH and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1ng/mL
  • Intended UseHuman MCT ELISA Kit allows for the in vitro quantitative determination of MCT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: MCH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCH antibody and an MCH-HRP conjugate. The assay sample and buffer are incubated together with MCH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCH concentration since MCH from samples and MCH-HRP conjugate compete for the anti-MCH antibody binding site. Since the number of sites is limited, as more sites are occupied by MCH from the sample, fewer sites are left to bind MCH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCH concentration in each sample is interpolated from this standard curve.



Inquiry About Human MCT ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!