SensitivityThe minimum detectable dose of human bCTx is typically less than 125 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human bCTx concentration that could be differentiated from zero. It was determined the me
Intended UseHuman bCTx ELISA Kit allows for the in vitro quantitative determination of bCTx , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to bCTx. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated bCTx. A competitive inhibition reaction is launched between bCTx (Standards or samples) and Biotin-conjugated bCTx with the pre-coated antibody specific for bCTx. The more amount of bCTx in samples, the less antibody bound by Biotin-conjugated bCTx. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of bCTx in the sample. The color development is stopped and the intensity of the color is measured.