-
Alternative name
Listeriolysin O ELISA KIT; hly ELISA KIT;
-
Catalog
E022890
-
species
Human
-
GeneLLO
-
SpecificityNo significant cross-reactivity or interference between Human LLO and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity1.0IU/ml.
-
Intended UseHuman LLO ELISA Kit allows for the in vitro quantitative determination of LLO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalIntroduction: This Quantitative Sandwich ELISA kit is in tended to determinate LLO concentrations in Human serum, plasma and other body fluids. Using Purified Human LLO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LLO and LLO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LLO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-