SpecificityThis assay has high sensitivity and excellent specificity for detection of ARbeta1Ab. No significant cross-reactivity or interference between ARbeta1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARbeta1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman b1AR ELISA Kit allows for the in vitro quantitative determination of b1AR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilySignal Transduction
Product Description specificalIntended Uses||This ARbeta1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARbeta1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: ARbeta1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing beta 1 Adrenergic Receptor antigen and an ARbeta1Ab-HRP conjugate. The assay sample and buffer are incubated together with ARbeta1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARbeta1Ab concentration since ARbeta1Ab from samples and ARbeta1Ab-HRP conjugate compete for the beta 1 Adrenergic Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by ARbeta1Ab from the sample, fewer sites are left to bind ARbeta1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARbeta1Ab concentration in each sample is interpolated from this standard curve.