Human LDB1 ELISA Kit

Alternative name
Human LDB-1 ELISA Kit;Human carboxyl-terminal LIM domain-binding protein 2 ELISA Kit;Human CLIM-2 ELISA Kit;Human LIM domain-binding factor CLIM2 ELISA Kit;Human hLdb1 ELISA Kit;Human nuclear LIM interactor ELISA Kit;Human CLIM2 ELISA Kit;Human NLI ELISA Kit;Human LIM domain binding 1 ELISA Kit;Human LIM domain-binding protein 1 ELISA Kit;Human LIM domain-binding factor-1 ELISA Kit;Human carboxy terminal LIM domain protein 2 ELISA Kit;
Catalog
E022712
species
Human
GeneLDB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of LIM Domain Binding Protein 1 (LDB1). No significant cross-reactivity or interference between LIM Domain Binding Protein 1 (LDB1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman LDB1 ELISA Kit allows for the in vitro quantitative determination of LDB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM Domain Binding Protein 1 (LDB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM Domain Binding Protein 1 (LDB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM Domain Binding Protein 1 (LDB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM Domain Binding Protein 1 (LDB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human LIM domain-binding protein 1 Protein information
Uniprot ID LDB1_HUMAN
Uniprot AC Q86U70; B4DUC4; O75479; O96010; Q1EQX1; Q9UGM4;
UniGene Hs.454418; Hs.672935;
GeneID 8861
KEGG hsa:8861;
Human LIM domain-binding protein 1 Protein SEQUENCE
SEQUENCE 411 AA; 46533 MW; 5BB5348A36044580 CRC64;
MSVGCACPGC SSKSFKLYSP KEPPNGNAFP PFHPGTMLDR DVGPTPMYPP
TYLEPGIGRH TPYGNQTDYR IFELNKRLQN WTEECDNLWW DAFTTEFFED
DAMLTITFCL EDGPKRYTIG RTLIPRYFRS IFEGGATELY YVLKHPKEAF
HSNFVSLDCD QGSMVTQHGK PMFTQVCVEG RLYLEFMFDD MMRIKTWHFS
IRQHRELIPR SILAMHAQDP QMLDQLSKNI TRCGLSNSTL NYLRLCVILE
PMQELMSRHK TYSLSPRDCL KTCLFQKWQR MVAPPAEPTR QQPSKRRKRK
MSGGSTMSSG GGNTNNSNSK KKSPASTFAL SSQVPDVMVV GEPTLMGGEF
GDEDERLITR LENTQFDAAN GIDDEDSFNN SPALGANSPW NSKPPSSQES
KSENPTSQAS Q
UCSC uc001kuk.6; human. [Q86U70-1];

Category

Human LDB1 ELISA Kit