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Alternative name
Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APPI ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid protein ELISA KIT; Cerebral vascular amyloid peptide ELISA KIT; CVAP ELISA KIT; PreA4 ELISA KIT; Protease nexin-II ELISA KIT; PN-IIAPP ELISA KIT; A4 ELISA KIT; AD1 ELISA KIT; APP ELISA KIT; CVAP ELISA KIT; PN-II ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT
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Catalog
E043697
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species
Mouse
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GeneAbeta42
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse Abeta42 ELISA Kit allows for the in vitro quantitative determination of Abeta42 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyNeurobiology
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Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This A? 42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse A? 42. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY A? 42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-A? 42 antibody and an A? 42-HRP conjugate. The assay sample and buffer are incubated together with A? 42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A? 42 concentration since A? 42 from samples and A? 42-HRP conjugate compete for the anti-A? 42 antibody binding site. Since the number of sites is limited, as more sites are occupied by A? 42 from the sample, fewer sites are left to bind A? 42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A? 42 concentration in each sample is interpolated from this standard curve.
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