Human LAP ELISA Kit

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  • Alternative name

    Cytosol aminopeptidase ELISA KIT; Leucine aminopeptidase 3 ELISA KIT; LAP-3 ELISA KIT; Leucyl aminopeptidase ELISA KIT; Peptidase S ELISA KIT; Proline aminopeptidase (EC:3.4.11.5) ELISA KIT; Prolyl aminopeptidaseLAP3 ELISA KIT; LAPEP ELISA KIT; PEPS ELISA KIT; LAP-3 ELISA KIT

  • Catalog
    E022388
  • species
    Human
  • GeneLAP
  • Standard CurveHuman LAP ELISA Kit
  • Other Species Mouse LAP ELISA KitBovine LAP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LAP. No significant cross-reactivity or interference between LAP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LAP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman LAP ELISA Kit allows for the in vitro quantitative determination of LAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This LAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LAP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LAP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LAP. Standards or samples are then added to the microtiter plate wells and LAP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LAP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LAP are added to each well to "sandwich" the LAP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LAP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LAP concentration in each sample is interpolated from this standard curve.


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