Human LTBP1 ELISA Kit

Alternative name
Human LTBP-1 ELISA Kit;Human transforming growth factor beta-1-binding protein 1 ELISA Kit;Human TGF-beta1-BP-1 ELISA Kit;Human latent transforming growth factor beta binding protein 1 ELISA Kit;Human latent-transforming growth factor beta-binding protein 1 ELISA Kit;
Catalog
E022231
species
Human
GeneLTBP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of LTBP-1. No significant cross-reactivity or interference between LTBP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTBP-1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman LTBP1 ELISA Kit allows for the in vitro quantitative determination of LTBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This LTBP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTBP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LTBP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTBP-1 antibody and an LTBP-1-HRP conjugate. The assay sample and buffer are incubated together with LTBP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTBP-1 concentration since LTBP-1 from samples and LTBP-1-HRP conjugate compete for the anti-LTBP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTBP-1 from the sample, fewer sites are left to bind LTBP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTBP-1 concentration in each sample is interpolated from this standard curve.
Human Latent-transforming growth factor beta-binding protein 1 Protein information
Uniprot ID LTBP1_HUMAN
Uniprot AC Q14766; A1L3V1; P22064; Q53SD8; Q53SF3; Q53SG1; Q59HF7; Q8TD95;
UniGene Hs.619315;
GeneID 4052
KEGG hsa:4052;
Human Latent-transforming growth factor beta-binding protein 1 Protein SEQUENCE
SEQUENCE 1721 AA; 186796 MW; 432489CAC3023754 CRC64;
MAGAWLRWGL LLWAGLLASS AHGRLRRITY VVHPGPGLAA GALPLSGPPR
SRTFNVALNA RYSRSSAAAG APSRASPGVP SERTRRTSKP GGAALQGLRP
PPPPPPEPAR PAVPGGQLHP NPGGHPAAAP FTKQGRQVVR SKVPQETQSG
GGSRLQVHQK QQLQGVNVCG GRCCHGWSKA PGSQRCTKPS CVPPCQNGGM
CLRPQLCVCK PGTKGKACET IAAQDTSSPV FGGQSPGAAS SWGPPEQAAK
HTSSKKADTL PRVSPVAQMT LTLKPKPSVG LPQQIHSQVT PLSSQSVVIH
HGQTQEYVLK PKYFPAQKGI SGEQSTEGSF PLRYVQDQVA APFQLSNHTG
RIKVVFTPSI CKVTCTKGSC QNSCEKGNTT TLISENGHAA DTLTATNFRV
VICHLPCMNG GQCSSRDKCQ CPPNFTGKLC QIPVHGASVP KLYQHSQQPG
KALGTHVIHS THTLPLTVTS QQGVKVKFPP NIVNIHVKHP PEASVQIHQV
SRIDGPTGQK TKEAQPGQSQ VSYQGLPVQK TQTIHSTYSH QQVIPHVYPV
AAKTQLGRCF QETIGSQCGK ALPGLSKQED CCGTVGTSWG FNKCQKCPKK
PSYHGYNQMM ECLPGYKRVN NTFCQDINEC QLQGVCPNGE CLNTMGSYRC
TCKIGFGPDP TFSSCVPDPP VISEEKGPCY RLVSSGRQCM HPLSVHLTKQ
LCCCSVGKAW GPHCEKCPLP GTAAFKEICP GGMGYTVSGV HRRRPIHHHV
GKGPVFVKPK NTQPVAKSTH PPPLPAKEEP VEALTFSREH GPGVAEPEVA
TAPPEKEIPS LDQEKTKLEP GQPQLSPGIS TIHLHPQFPV VIEKTSPPVP
VEVAPEASTS SASQVIAPTQ VTEINECTVN PDICGAGHCI NLPVRYTCIC
YEGYRFSEQQ RKCVDIDECT QVQHLCSQGR CENTEGSFLC ICPAGFMASE
EGTNCIDVDE CLRPDVCGEG HCVNTVGAFR CEYCDSGYRM TQRGRCEDID
ECLNPSTCPD EQCVNSPGSY QCVPCTEGFR GWNGQCLDVD ECLEPNVCAN
GDCSNLEGSY MCSCHKGYTR TPDHKHCRDI DECQQGNLCV NGQCKNTEGS
FRCTCGQGYQ LSAAKDQCED IDECQHRHLC AHGQCRNTEG SFQCVCDQGY
RASGLGDHCE DINECLEDKS VCQRGDCINT AGSYDCTCPD GFQLDDNKTC
QDINECEHPG LCGPQGECLN TEGSFHCVCQ QGFSISADGR TCEDIDECVN
NTVCDSHGFC DNTAGSFRCL CYQGFQAPQD GQGCVDVNEC ELLSGVCGEA
FCENVEGSFL CVCADENQEY SPMTGQCRSR TSTDLDVDVD QPKEEKKECY
YNLNDASLCD NVLAPNVTKQ ECCCTSGVGW GDNCEIFPCP VLGTAEFTEM
CPKGKGFVPA GESSSEAGGE NYKDADECLL FGQEICKNGF CLNTRPGYEC
YCKQGTYYDP VKLQCFDMDE CQDPSSCIDG QCVNTEGSYN CFCTHPMVLD
ASEKRCIRPA ESNEQIEETD VYQDLCWEHL SDEYVCSRPL VGKQTTYTEC
CCLYGEAWGM QCALCPLKDS DDYAQLCNIP VTGRRQPYGR DALVDFSEQY
TPEADPYFIQ DRFLNSFEEL QAEECGILNG CENGRCVRVQ EGYTCDCFDG
YHLDTAKMTC VDVNECDELN NRMSLCKNAK CINTDGSYKC LCLPGYVPSD
KPNYCTPLNT ALNLEKDSDL E
UCSC uc002rou.4; human. [Q14766-1];

Category

Human LTBP1 ELISA Kit

Human LTBP-1 ELISA Kit;Human transforming growth factor beta-1-binding protein 1 ELISA Kit;Human TGF-beta1-BP-1 ELISA Kit;Human latent transforming growth factor beta binding protein 1 ELISA Kit;Human latent-transforming growth factor beta-binding protein 1 ELISA Kit;