Human LTBP1 ELISA Kit

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  • Alternative name

    Human LTBP-1 ELISA Kit;Human transforming growth factor beta-1-binding protein 1 ELISA Kit;Human TGF-beta1-BP-1 ELISA Kit;Human latent transforming growth factor beta binding protein 1 ELISA Kit;Human latent-transforming growth factor beta-binding protein 1 ELISA Kit;

  • Catalog
    E022231
  • species
    Human
  • GeneLTBP1
  • Standard CurveHuman LTBP1 ELISA Kit
  • Other Species Mouse Ltbp1 ELISA KitRat Ltbp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LTBP-1. No significant cross-reactivity or interference between LTBP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTBP-1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman LTBP1 ELISA Kit allows for the in vitro quantitative determination of LTBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This LTBP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTBP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LTBP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTBP-1 antibody and an LTBP-1-HRP conjugate. The assay sample and buffer are incubated together with LTBP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTBP-1 concentration since LTBP-1 from samples and LTBP-1-HRP conjugate compete for the anti-LTBP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTBP-1 from the sample, fewer sites are left to bind LTBP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTBP-1 concentration in each sample is interpolated from this standard curve.
  • Human Latent-transforming growth factor beta-binding protein 1 Protein information
  • Uniprot ID LTBP1_HUMAN
  • Uniprot AC Q14766; A1L3V1; P22064; Q53SD8; Q53SF3; Q53SG1; Q59HF7; Q8TD95;
  • UniGene Hs.619315;
  • GeneID 4052
  • KEGG hsa:4052;
  • Human Latent-transforming growth factor beta-binding protein 1 Protein SEQUENCE
  • SEQUENCE 1721 AA; 186796 MW; 432489CAC3023754 CRC64;

    MAGAWLRWGL LLWAGLLASS AHGRLRRITY VVHPGPGLAA GALPLSGPPR

    SRTFNVALNA RYSRSSAAAG APSRASPGVP SERTRRTSKP GGAALQGLRP

    PPPPPPEPAR PAVPGGQLHP NPGGHPAAAP FTKQGRQVVR SKVPQETQSG

    GGSRLQVHQK QQLQGVNVCG GRCCHGWSKA PGSQRCTKPS CVPPCQNGGM

    CLRPQLCVCK PGTKGKACET IAAQDTSSPV FGGQSPGAAS SWGPPEQAAK

    HTSSKKADTL PRVSPVAQMT LTLKPKPSVG LPQQIHSQVT PLSSQSVVIH

    HGQTQEYVLK PKYFPAQKGI SGEQSTEGSF PLRYVQDQVA APFQLSNHTG

    RIKVVFTPSI CKVTCTKGSC QNSCEKGNTT TLISENGHAA DTLTATNFRV

    VICHLPCMNG GQCSSRDKCQ CPPNFTGKLC QIPVHGASVP KLYQHSQQPG

    KALGTHVIHS THTLPLTVTS QQGVKVKFPP NIVNIHVKHP PEASVQIHQV

    SRIDGPTGQK TKEAQPGQSQ VSYQGLPVQK TQTIHSTYSH QQVIPHVYPV

    AAKTQLGRCF QETIGSQCGK ALPGLSKQED CCGTVGTSWG FNKCQKCPKK

    PSYHGYNQMM ECLPGYKRVN NTFCQDINEC QLQGVCPNGE CLNTMGSYRC

    TCKIGFGPDP TFSSCVPDPP VISEEKGPCY RLVSSGRQCM HPLSVHLTKQ

    LCCCSVGKAW GPHCEKCPLP GTAAFKEICP GGMGYTVSGV HRRRPIHHHV

    GKGPVFVKPK NTQPVAKSTH PPPLPAKEEP VEALTFSREH GPGVAEPEVA

    TAPPEKEIPS LDQEKTKLEP GQPQLSPGIS TIHLHPQFPV VIEKTSPPVP

    VEVAPEASTS SASQVIAPTQ VTEINECTVN PDICGAGHCI NLPVRYTCIC

    YEGYRFSEQQ RKCVDIDECT QVQHLCSQGR CENTEGSFLC ICPAGFMASE

    EGTNCIDVDE CLRPDVCGEG HCVNTVGAFR CEYCDSGYRM TQRGRCEDID

    ECLNPSTCPD EQCVNSPGSY QCVPCTEGFR GWNGQCLDVD ECLEPNVCAN

    GDCSNLEGSY MCSCHKGYTR TPDHKHCRDI DECQQGNLCV NGQCKNTEGS

    FRCTCGQGYQ LSAAKDQCED IDECQHRHLC AHGQCRNTEG SFQCVCDQGY

    RASGLGDHCE DINECLEDKS VCQRGDCINT AGSYDCTCPD GFQLDDNKTC

    QDINECEHPG LCGPQGECLN TEGSFHCVCQ QGFSISADGR TCEDIDECVN

    NTVCDSHGFC DNTAGSFRCL CYQGFQAPQD GQGCVDVNEC ELLSGVCGEA

    FCENVEGSFL CVCADENQEY SPMTGQCRSR TSTDLDVDVD QPKEEKKECY

    YNLNDASLCD NVLAPNVTKQ ECCCTSGVGW GDNCEIFPCP VLGTAEFTEM

    CPKGKGFVPA GESSSEAGGE NYKDADECLL FGQEICKNGF CLNTRPGYEC

    YCKQGTYYDP VKLQCFDMDE CQDPSSCIDG QCVNTEGSYN CFCTHPMVLD

    ASEKRCIRPA ESNEQIEETD VYQDLCWEHL SDEYVCSRPL VGKQTTYTEC

    CCLYGEAWGM QCALCPLKDS DDYAQLCNIP VTGRRQPYGR DALVDFSEQY

    TPEADPYFIQ DRFLNSFEEL QAEECGILNG CENGRCVRVQ EGYTCDCFDG

    YHLDTAKMTC VDVNECDELN NRMSLCKNAK CINTDGSYKC LCLPGYVPSD

    KPNYCTPLNT ALNLEKDSDL E

  • UCSC uc002rou.4; human. [Q14766-1];



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