Mouse VS ELISA Kit

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  • Alternative name

    Versican core protein ELISA KIT; Chondroitin sulfate proteoglycan core protein 2 ELISA KIT; Chondroitin sulfate proteoglycan 2 ELISA KIT; Glial hyaluronate-binding protein ELISA KIT; GHAP ELISA KIT; Large fibroblast proteoglycan ELISA KIT; PG-MVCAN ELISA KIT; CSPG2 ELISA KIT; Chondroitin sulfate proteoglycan 2 ELISA KIT; GHAP ELISA KIT

  • Catalog
    E072458
  • species
    Mouse
  • GeneVS
  • Standard CurveMouse VS ELISA Kit
  • Other Species Human VS ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Versican (VS). No significant cross-reactivity or interference between Versican (VS) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseMouse VS ELISA Kit allows for the in vitro quantitative determination of VS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Versican (VS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Versican (VS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Versican (VS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Versican (VS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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