Human LELP1 ELISA Kit

Alternative name
Human Novel small proline-rich protein ELISA Kit;Human late cornified envelope like proline rich 1 ELISA Kit;Human late cornified envelope-like proline-rich protein 1 ELISA Kit;Human novel small proline rich protein ELISA Kit;
Catalog
E022222
species
Human
GeneLELP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Late Cornified Envelope Like Proline Rich Protein 1 (LELP1). No significant cross-reactivity or interference between Late Cornified Envelope Like Proline Rich Protein 1 (LELP1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.54ng/mL.
Intended UseHuman LELP1 ELISA Kit allows for the in vitro quantitative determination of LELP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Late Cornified Envelope Like Proline Rich Protein 1 (LELP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Late Cornified Envelope Like Proline Rich Protein 1 (LELP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Late Cornified Envelope Like Proline Rich Protein 1 (LELP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Late Cornified Envelope Like Proline Rich Protein 1 (LELP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Late cornified envelope-like proline-rich protein 1 Protein information
Uniprot ID LELP1_HUMAN
Uniprot AC Q5T871; A1L4E1;
UniGene Hs.125785;
GeneID 149018
KEGG hsa:149018;
Human Late cornified envelope-like proline-rich protein 1 Protein SEQUENCE
SEQUENCE 98 AA; 10697 MW; 473BDFEB85D3EEA5 CRC64;
MSSDDKSKSN DPKTEPKNCD PKCEQKCESK CQPSCLKKLL QRCFEKCPWE
KCPAPPKCLP CPSQSPSSCP PQPCTKPCPP KCPSSCPHAC PPPCPPPE
UCSC uc001fbl.5; human.;

Category

Human LELP1 ELISA Kit

Human Novel small proline-rich protein ELISA Kit;Human late cornified envelope like proline rich 1 ELISA Kit;Human late cornified envelope-like proline-rich protein 1 ELISA Kit;Human novel small proline rich protein ELISA Kit;