Human LELP1 ELISA Kit

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  • Alternative name

    Human Novel small proline-rich protein ELISA Kit;Human late cornified envelope like proline rich 1 ELISA Kit;Human late cornified envelope-like proline-rich protein 1 ELISA Kit;Human novel small proline rich protein ELISA Kit;

  • Catalog
    E022222
  • species
    Human
  • GeneLELP1
  • Standard CurveHuman LELP1 ELISA Kit
  • Other Species Mouse Lelp1 ELISA KitBovine LELP1 ELISA KitMonkey LELP1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Late Cornified Envelope Like Proline Rich Protein 1 (LELP1). No significant cross-reactivity or interference between Late Cornified Envelope Like Proline Rich Protein 1 (LELP1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.54ng/mL.
  • Intended UseHuman LELP1 ELISA Kit allows for the in vitro quantitative determination of LELP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Late Cornified Envelope Like Proline Rich Protein 1 (LELP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Late Cornified Envelope Like Proline Rich Protein 1 (LELP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Late Cornified Envelope Like Proline Rich Protein 1 (LELP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Late Cornified Envelope Like Proline Rich Protein 1 (LELP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Late cornified envelope-like proline-rich protein 1 Protein information
  • Uniprot ID LELP1_HUMAN
  • Uniprot AC Q5T871; A1L4E1;
  • UniGene Hs.125785;
  • GeneID 149018
  • KEGG hsa:149018;
  • Human Late cornified envelope-like proline-rich protein 1 Protein SEQUENCE
  • SEQUENCE 98 AA; 10697 MW; 473BDFEB85D3EEA5 CRC64;

    MSSDDKSKSN DPKTEPKNCD PKCEQKCESK CQPSCLKKLL QRCFEKCPWE

    KCPAPPKCLP CPSQSPSSCP PQPCTKPCPP KCPSSCPHAC PPPCPPPE

  • UCSC uc001fbl.5; human.;



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