Human CYP51A1 ELISA Kit

Alternative name
Human LDM ELISA Kit;Human CYPLI ELISA Kit;Human cytochrome P450 51A1 ELISA Kit;Human cytochrome P450-14DM ELISA Kit;Human cytochrome P45014DM ELISA Kit;Human cytochrome P450LI ELISA Kit;Human sterol 14-alpha demethylase ELISA Kit;Human CYP51 ELISA Kit;Human CP51 ELISA Kit;Human CYPL1 ELISA Kit;Human P450-14DM ELISA Kit;Human P450L1 ELISA Kit;Human cytochrome P450 family 51 subfamily A member 1 ELISA Kit;Human lanosterol 14-alpha demethylase ELISA Kit;Human cytochrome P450, 51 (lanosterol 14-alpha-demethylase) ELISA Kit;Human cytochrome P450, family 51, subfamily A, polypeptide 1 ELISA Kit;
Catalog
E022195
species
Human
GeneCYP51A1
SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP51A1. No significant cross-reactivity or interference between CYP51A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP51A1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman CYP51A1 ELISA Kit allows for the in vitro quantitative determination of CYP51A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This CYP51A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYP51A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CYP51A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CYP51A1 antibody and an CYP51A1-HRP conjugate. The assay sample and buffer are incubated together with CYP51A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP51A1 concentration since CYP51A1 from samples and CYP51A1-HRP conjugate compete for the anti-CYP51A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP51A1 from the sample, fewer sites are left to bind CYP51A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP51A1 concentration in each sample is interpolated from this standard curve.
Human Lanosterol 14-alpha demethylase Protein information
Uniprot ID CP51A_HUMAN
Uniprot AC Q99868;
UniGene Hs.417077;
GeneID 1595
KEGG hsa:1595;
Human Lanosterol 14-alpha demethylase Protein SEQUENCE
SEQUENCE 503 AA; 56806 MW; 4FCEC147FB4DED86 CRC64;
MLLLGLLQAG GSVLGQAMEK VTGGNLLSML LIACAFTLSL VYLIRLAAGH
LVQLPAGVKS PPYIFSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM
VGKTFTYLLG SDAAALLFNS KNEDLNAEDV YSRLTTPVFG KGVAYDVPNP
VFLEQKKMLK SGLNIAHFKQ HVSIIEKETK EYFESWGESG EKNVFEALSE
LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL LPGWLPLPSF
RRRDRAHREI KDIFYKAIQK RRQSQEKIDD ILQTLLDATY KDGRPLTDDE
VAGMLIGLLL AGQHTSSTTS AWMGFFLARD KTLQKKCYLE QKTVCGENLP
PLTYDQLKDL NLLDRCIKET LRLRPPIMIM MRMARTPQTV AGYTIPPGHQ
VCVSPTVNQR LKDSWVERLD FNPDRYLQDN PASGEKFAYV PFGAGRHRCI
GENFAYVQIK TIWSTMLRLY EFDLIDGYFP TVNYTTMIHT PENPVIRYKR
RSK
UCSC uc003ulm.5; human. [Q16850-1];

Category

Human CYP51A1 ELISA Kit