Human ANGPTL1 ELISA Kit

Alternative name
Human Angiopoietin-3 ELISA Kit;Human ANG-3 ELISA Kit;Human angiopoietin-like protein 1 ELISA Kit;Human ANG3 ELISA Kit;Human ANGPT3 ELISA Kit;Human ARP1 ELISA Kit;Human AngY ELISA Kit;Human UNQ162 ELISA Kit;Human dJ595C2.2 ELISA Kit;Human angiopoietin like 1 ELISA Kit;Human angiopoietin-related protein 1 ELISA Kit;Human angioarrestin ELISA Kit;Human angiopoietin 3 ELISA Kit;Human angiopoietin Y1 ELISA Kit;Human dJ595C2.2 (angiopoietin Y1) ELISA Kit;
Catalog
E002059
species
Human
GeneANGPTL1
Standard Curve
Other Species Mouse Angptl1 ELISA Kit Bovine ANGPTL1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 1 (ANGPTL1). No significant cross-reactivity or interference between Angiopoietin Like Protein 1 (ANGPTL1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman ANGPTL1 ELISA Kit allows for the in vitro quantitative determination of ANGPTL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 1 (ANGPTL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 1 (ANGPTL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 1 (ANGPTL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 1 (ANGPTL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Angiopoietin-related protein 1 Protein information
Uniprot ID ANGL1_HUMAN
Uniprot AC O95841; Q5T5Z5;
UniGene Hs.591474;
GeneID 9068
KEGG hsa:9068;
Human Angiopoietin-related protein 1 Protein SEQUENCE
SEQUENCE 491 AA; 56720 MW; 3C4DB8DEF6CF7E99 CRC64;
MKTFTWTLGV LFFLLVDTGH CRGGQFKIKK INQRRYPRAT DGKEEAKKCA
YTFLVPEQRI TGPICVNTKG QDASTIKDMI TRMDLENLKD VLSRQKREID
VLQLVVDVDG NIVNEVKLLR KESRNMNSRV TQLYMQLLHE IIRKRDNSLE
LSQLENKILN VTTEMLKMAT RYRELEVKYA SLTDLVNNQS VMITLLEEQC
LRIFSRQDTH VSPPLVQVVP QHIPNSQQYT PGLLGGNEIQ RDPGYPRDLM
PPPDLATSPT KSPFKIPPVT FINEGPFKDC QQAKEAGHSV SGIYMIKPEN
SNGPMQLWCE NSLDPGGWTV IQKRTDGSVN FFRNWENYKK GFGNIDGEYW
LGLENIYMLS NQDNYKLLIE LEDWSDKKVY AEYSSFRLEP ESEFYRLRLG
TYQGNAGDSM MWHNGKQFTT LDRDKDMYAG NCAHFHKGGW WYNACAHSNL
NGVWYRGGHY RSKHQDGIFW AEYRGGSYSL RAVQMMIKPI D
UCSC uc001gma.4; human.;

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Human ANGPTL1 ELISA Kit