Human ATM ELISA Kit

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  • Alternative name

    Serine-protein kinase ATM ELISA KIT; Ataxia telangiectasia mutated ELISA KIT; A-T mutatedATM ELISA KIT; A-T mutated ELISA KIT

  • Catalog
    E003897
  • species
    Human
  • GeneATM
  • Standard CurveHuman ATM ELISA Kit
  • Other Species Mouse Atm ELISA KitPorcine ATM ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ataxia Telangiectasia Mutated (ATM). No significant cross-reactivity or interference between Ataxia Telangiectasia Mutated (ATM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.8pg/mL.
  • Intended UseHuman ATM ELISA Kit allows for the in vitro quantitative determination of ATM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxia Telangiectasia Mutated (ATM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ataxia Telangiectasia Mutated (ATM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ataxia Telangiectasia Mutated (ATM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ataxia Telangiectasia Mutated (ATM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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