Human FIL1h ELISA Kit

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  • Alternative name

    Interleukin-36 beta ELISA KIT; FIL1 eta ELISA KIT; Interleukin-1 eta ELISA KIT; IL-1 eta ELISA KIT; Interleukin-1 family member 8 ELISA KIT; IL-1F8 ELISA KIT; Interleukin-1 homolog 2 ELISA KIT; IL-1H2IL36B ELISA KIT; IL1F8 ELISA KIT; IL1H2 ELISA KIT; IL-1 eta ELISA KIT; IL-1F8 ELISA KIT; IL-1H2 ELISA KIT

  • Catalog
    E020420
  • species
    Human
  • GeneFIL1h
  • Standard CurveHuman FIL1h ELISA Kit
  • Other Species Mouse FIL1h ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman FIL1h ELISA Kit allows for the in vitro quantitative determination of FIL1h , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: FIL1t ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FIL1t. Standards or samples are then added to the microtiter plate wells and FIL1t if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FIL1t present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FIL1t are added to each well to "sandwich" the FIL1t immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FIL1t and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FIL1t concentration in each sample is interpolated from this standard curve.



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