Human SERPINB10 ELISA Kit

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  • Alternative name

    Human bomapin ELISA Kit;Human peptidase inhibitor 10 ELISA Kit;Human PI-10 ELISA Kit;Human PI10 ELISA Kit;Human serpin family B member 10 ELISA Kit;Human serpin B10 ELISA Kit;Human protease inhibitor 10 (ovalbumin type, bomapin) ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 10 ELISA Kit;Human serpin peptidase inhibitor, clade B (ovalbumin), member 10 ELISA Kit;

  • Catalog
    E034864
  • species
    Human
  • GeneSERPINB10
  • Standard CurveHuman SERPINB10 ELISA Kit
  • Other Species Mouse Serpinb10 ELISA KitBovine SERPINB10 ELISA KitChicken SERPINB10 ELISA KitRat Serpinb10 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SERPINB10. No significant cross-reactivity or interference between SERPINB10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SERPINB10 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SERPINB10 ELISA Kit allows for the in vitro quantitative determination of SERPINB10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the Assay: SERPINB10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SERPINB10 antibody and an SERPINB10-HRP conjugate. The assay sample and buffer are incubated together with SERPINB10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERPINB10 concentration since SERPINB10 from samples and SERPINB10-HRP conjugate compete for the anti-SERPINB10 antibody binding site. Since the number of sites is limited, as more sites are occupied by SERPINB10 from the sample, fewer sites are left to bind SERPINB10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERPINB10 concentration in each sample is interpolated from this standard curve.
  • Human Serpin B10 Protein information
  • Uniprot ID SPB10_HUMAN
  • Uniprot AC P48595; Q4VAX4; Q4VAX7;
  • UniGene Hs.158339;
  • GeneID 5273
  • KEGG hsa:5273;
  • Human Serpin B10 Protein SEQUENCE
  • SEQUENCE 397 AA; 45403 MW; 8CE01246867154DF CRC64;

    MDSLATSINQ FALELSKKLA ESAQGKNIFF SSWSISTSLT IVYLGAKGTT

    AAQMAQVLQF NRDQGVKCDP ESEKKRKMEF NLSNSEEIHS DFQTLISEIL

    KPNDDYLLKT ANAIYGEKTY AFHNKYLEDM KTYFGAEPQP VNFVEASDQI

    RKDINSWVER QTEGKIQNLL PDDSVDSTTR MILVNALYFK GIWEHQFLVQ

    NTTEKPFRIN ETTSKPVQMM FMKKKLHIFH IEKPKAVGLQ LYYKSRDLSL

    LILLPEDING LEQLEKAITY EKLNEWTSAD MMELYEVQLH LPKFKLEDSY

    DLKSTLSSMG MSDAFSQSKA DFSGMSSARN LFLSNVFHKA FVEINEQGTE

    AAAGSGSEID IRIRVPSIEF NANHPFLFFI RHNKTNTILF YGRLCSP

  • UCSC uc010xev.3; human.;


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