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Alternative name
C-X-C motif chemokine 11 ELISA KIT; Beta-R1 ELISA KIT; H174 ELISA KIT; Interferon gamma-inducible protein 9 ELISA KIT; IP-9 ELISA KIT; Interferon-inducible T-cell alpha chemoattractant ELISA KIT; I-TAC ELISA KIT; Small-inducible cytokine B11CXCL11 ELISA KIT; ITAC ELISA KIT; SCYB11 ELISA KIT; SCYB9B ELISA KIT; IP-9 ELISA KIT; I-TAC ELISA KIT
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Catalog
E020313
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species
Human
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GeneITaC
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC).
No significant cross-reactivity or interference between Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 25.8pg/mL.
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Intended UseHuman ITaC ELISA Kit allows for the in vitro quantitative determination of ITaC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible T-Cell Alpha Chemoattractant (ITaC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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