Human NANOG ELISA Kit

Alternative name
Human homeobox transcription factor Nanog ELISA Kit;Human hNanog ELISA Kit;Human Nanog homeobox ELISA Kit;Human homeobox protein NANOG ELISA Kit;Human homeobox transcription factor Nanog-delta 48 ELISA Kit;
Catalog
E025839
species
Human
GeneNANOG
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NANOG. No significant cross-reactivity or interference between human NANOG and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NANOG is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman NANOG ELISA Kit allows for the in vitro quantitative determination of NANOG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NANOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NANOG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NANOG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NANOG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Homeobox protein NANOG Protein information
Uniprot ID NANOG_HUMAN
Uniprot AC Q9H9S0; D3DUU4; Q2TTG0; Q6JZS5;
UniGene Hs.635882;
GeneID 79923
KEGG hsa:79923;
Human Homeobox protein NANOG Protein SEQUENCE
SEQUENCE 305 AA; 34620 MW; AC3B2E067C591773 CRC64;
MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET
VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT
VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS
KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT
WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS
CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM
QPEDV
UCSC uc009zfy.2; human. [Q9H9S0-1];

Category

Human NANOG ELISA Kit

Human homeobox transcription factor Nanog ELISA Kit;Human hNanog ELISA Kit;Human Nanog homeobox ELISA Kit;Human homeobox protein NANOG ELISA Kit;Human homeobox transcription factor Nanog-delta 48 ELISA Kit;