Human IGF2AS ELISA Kit

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  • Alternative name

    Putative insulin-like growth factor 2 antisense gene protein ELISA KIT; IGF2 antisense RNA 1 ELISA KIT; IGF2 antisense gene protein 1 ELISA KIT; PEG8/IGF2AS protein ELISA KIT; Putative insulin-like growth factor 2 antisense gene protein 1 ELISA KIT; IGF2-AS1IGF2-AS ELISA KIT; IGF2-AS1 ELISA KIT; IGF2AS ELISA KIT; IGF2-AS1 ELISA KIT

  • Catalog
    E019933
  • species
    Human
  • GeneIGF2AS
  • Standard CurveHuman IGF2AS ELISA Kit
  • Other Species Mouse IGF2AS ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman IGF2AS ELISA Kit allows for the in vitro quantitative determination of IGF2AS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This IGF2AS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IGF2AS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY IGF2AS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF2AS antibody and an IGF2AS-HRP conjugate. The assay sample and buffer are incubated together with IGF2AS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF2AS concentration since IGF2AS from samples and IGF2AS-HRP conjugate compete for the anti-IGF2AS antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF2AS from the sample, fewer sites are left to bind IGF2AS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF2AS concentration in each sample is interpolated from this standard curve.



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