Mouse Pcsk9 ELISA Kit

Alternative name
Mouse Neural apoptosis-regulated convertase 1 ELISA Kit;Mouse NARC-1 ELISA Kit;Mouse proprotein convertase 9 ELISA Kit;Mouse PC9 ELISA Kit;Mouse subtilisin/kexin-like protease PC9 ELISA Kit;Mouse Narc1 ELISA Kit;Mouse AI415265 ELISA Kit;Mouse AI747682 ELISA Kit;Mouse FH3 ELISA Kit;Mouse HCHOLA3 ELISA Kit;Mouse proprotein convertase subtilisin/kexin type 9 ELISA Kit;Mouse convertase subtilisin ELISA Kit;Mouse neural apoptosis regulated convertase 1 ELISA Kit;Mouse proprotein convertase PC9 ELISA Kit;
Catalog
E063064
species
Mouse
GenePcsk9
Other Species Human PCSK9 ELISA Kit Rat Pcsk9 ELISA Kit
SpecificityNatural and recombinant mouse PCSK9
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Pcsk9 ELISA Kit allows for the in vitro quantitative determination of Pcsk9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse PCSK9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PCSK9 has been precoated onto 96-well plates. Standards(NSO, S156Q-694) from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PCSK9 amount of sample captured in plate. Background: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins (LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Mouse Proprotein convertase subtilisin/kexin type 9 Protein information
Uniprot ID PCSK9_MOUSE
Uniprot AC Q80W65; B1AZI4; Q3UEH7; Q8BXW9; Q8CFT6;
UniGene Mm.133268;
GeneID 100102
KEGG mmu:100102;
Mouse Proprotein convertase subtilisin/kexin type 9 Protein SEQUENCE
SEQUENCE 694 AA; 74823 MW; 977BD4BD1FAF98C0 CRC64;
MGTHCSAWLR WPLLPLLPPL LLLLLLLCPT GAGAQDEDGD YEELMLALPS
QEDGLADEAA HVATATFRRC SKEAWRLPGT YIVVLMEETQ RLQIEQTAHR
LQTRAARRGY VIKVLHIFYD LFPGFLVKMS SDLLGLALKL PHVEYIEEDS
FVFAQSIPWN LERIIPAWHQ TEEDRSPDGS SQVEVYLLDT SIQGAHREIE
GRVTITDFNS VPEEDGTRFH RQASKCDSHG THLAGVVSGR DAGVAKGTSL
HSLRVLNCQG KGTVSGTLIG LEFIRKSQLI QPSGPLVVLL PLAGGYSRIL
NAACRHLART GVVLVAAAGN FRDDACLYSP ASAPEVITVG ATNAQDQPVT
LGTLGTNFGR CVDLFAPGKD IIGASSDCST CFMSQSGTSQ AAAHVAGIVA
RMLSREPTLT LAELRQRLIH FSTKDVINMA WFPEDQQVLT PNLVATLPPS
THETGGQLLC RTVWSAHSGP TRTATATARC APEEELLSCS SFSRSGRRRG
DWIEAIGGQQ VCKALNAFGG EGVYAVARCC LVPRANCSIH NTPAARAGLE
THVHCHQKDH VLTGCSFHWE VEDLSVRRQP ALRSRRQPGQ CVGHQAASVY
ASCCHAPGLE CKIKEHGISG PSEQVTVACE AGWTLTGCNV LPGASLTLGA
YSVDNLCVAR VHDTARADRT SGEATVAAAI CCRSRPSAKA SWVQ
UCSC uc008tyi.2; mouse.;

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Mouse Pcsk9 ELISA Kit