Human IL9 ELISA Kit

Alternative name
Human IL-9 ELISA Kit;Human cytokine P40 ELISA Kit;Human T-cell growth factor p40 ELISA Kit;Human HP40 ELISA Kit;Human P40 ELISA Kit;Human interleukin 9 ELISA Kit;Human interleukin-9 ELISA Kit;Human homolog of mouse T cell and mast cell growth factor 40 ELISA Kit;Human p40 T-cell and mast cell growth factor ELISA Kit;Human p40 cytokine ELISA Kit;
Catalog
E019523
species
Human
GeneIL9
SpecificityThe Human IL-9 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-9 proteins within the range of 3.9 pg/ml - 250 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity2 pg/ml.
Intended UseHuman IL9 ELISA Kit allows for the in vitro quantitative determination of IL9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Human IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLV-I) transformed T cell line that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, M07e. This human cytokine and its murine homologue are now designated as human and mouse IL-9. The gene for H IL-9 has been mapped to human chromosome 5. As in the mouse system, the human IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteinerich protein with a predicted molecular mass of 14 kDa. Human IL-9 contains four potential Nlinked glycosylation sites and the native HIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although murine IL-9 is active on human cells, human IL-9 is not active on mouse cells. Human and murine IL9 are also capable of enhancing in vitro survival of human T cell lines as well as synergizing with Epo to support erythroid colony formation in vitro.
Human Interleukin-9 Protein information
Uniprot ID IL9_HUMAN
Uniprot AC P15248;
UniGene Hs.960;
GeneID 3578
KEGG hsa:3578;
Human Interleukin-9 Protein SEQUENCE
SEQUENCE 144 AA; 15909 MW; 2EAA08690558ECAB CRC64;
MLLAMVLTSA LLLCSVAGQG CPTLAGILDI NFLINKMQED PASKCHCSAN
VTSCLCLGIP SDNCTRPCFS ERLSQMTNTT MQTRYPLIFS RVKKSVEVLK
NNKCPYFSCE QPCNQTTAGN ALTFLKSLLE IFQKEKMRGM RGKI
UCSC uc003lbb.1; human.;

Human IL9 ELISA Kit

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