Human ZFAND5 ELISA Kit

Alternative name
Human zinc finger A20 domain-containing protein 2 ELISA Kit;Human zinc finger protein 216 ELISA Kit;Human ZA20D2 ELISA Kit;Human ZNF216 ELISA Kit;Human ZFAND5A ELISA Kit;Human zinc finger AN1-type containing 5 ELISA Kit;Human AN1-type zinc finger protein 5 ELISA Kit;Human zinc finger, A20 domain containing 2 ELISA Kit;Human zinc finger, AN1-type domain 5 ELISA Kit;
Catalog
E001937
species
Human
GeneZFAND5
SpecificityThis assay has high sensitivity and excellent specificity for detection of ZFAND5. No significant cross-reactivity or interference between ZFAND5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ZFAND5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL
Intended UseHuman ZFAND5 ELISA Kit allows for the in vitro quantitative determination of ZFAND5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This ZFAND5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZFAND5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ZFAND5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ZFAND5 antibody and an ZFAND5-HRP conjugate. The assay sample and buffer are incubated together with ZFAND5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZFAND5 concentration since ZFAND5 from samples and ZFAND5-HRP conjugate compete for the anti-ZFAND5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZFAND5 from the sample, fewer sites are left to bind ZFAND5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZFAND5 concentration in each sample is interpolated from this standard curve.
Human AN1-type zinc finger protein 5 Protein information
Uniprot ID ZFAN5_HUMAN
Uniprot AC O76080; A8K484;
UniGene Hs.406096;
GeneID 7763
KEGG hsa:7763;
Human AN1-type zinc finger protein 5 Protein SEQUENCE
SEQUENCE 213 AA; 23132 MW; E477504B1BA77753 CRC64;
MAQETNQTPG PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QQNSGRMSPM
GTASGSNSPT SDSASVQRAD TSLNNCEGAA GSTSEKSRNV PVAALPVTQQ
MTEMSISRED KITTPKTEVS EPVVTQPSPS VSQPSTSQSE EKAPELPKPK
KNRCFMCRKK VGLTGFDCRC GNLFCGLHRY SDKHNCPYDY KAEAAAKIRK
ENPVVVAEKI QRI
UCSC uc004aix.3; human.;

Human ZFAND5 ELISA Kit

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