Mouse Zic1 ELISA Kit

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  • Alternative name

    Mouse zinc finger protein of the cerebellum 1 ELISA Kit;Mouse ZIC ELISA Kit;Mouse ZNF201 ELISA Kit;Mouse zinc finger protein ZIC 1 ELISA Kit;Mouse Zic family member 1 (odd-paired homolog, Drosophila) ELISA Kit;Mouse odd-paired homolog ELISA Kit;

  • Catalog
    E073266
  • species
    Mouse
  • GeneZic1
  • Other Species Chicken ZIC1 ELISA KitHuman ZIC1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ZIC1. No significant cross-reactivity or interference between ZIC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ZIC1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Zic1 ELISA Kit allows for the in vitro quantitative determination of Zic1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the Assay: ZIC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZIC1 antibody and an ZIC1-HRP conjugate. The assay sample and buffer are incubated together with ZIC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZIC1 concentration since ZIC1 from samples and ZIC1-HRP conjugate compete for the anti-ZIC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZIC1 from the sample, fewer sites are left to bind ZIC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZIC1 concentration in each sample is interpolated from this standard curve.
  • Mouse Zinc finger protein ZIC 1 Protein information
  • Uniprot ID ZIC1_MOUSE
  • Uniprot AC P46684; Q6PAK5; Q80Y18;
  • UniGene Mm.335350; Mm.477226;
  • GeneID 22771
  • KEGG mmu:22771;
  • Mouse Zinc finger protein ZIC 1 Protein SEQUENCE
  • SEQUENCE 447 AA; 48331 MW; D41F412F44C2AD3D CRC64;

    MLLDAGPQYP AIGVTTFGAS RHHSAGDVAE RDVGLGINPF ADGMGAFKLN

    PSSHELASAG QTAFTSQAPG YAAAAALGHH HHPGHVGSYS SAAFNSTRDF

    LFRNRGFGDA AAAASAQHSL FAASAGGFGG PHGHTDAAGH LLFSGLHEQA

    AGHASPNVVN GQMRLGFSGD MYPRPEQYGQ VTSPRSEHYA APQLHGYGPM

    NVNMAAHHGA GAFFRYMRQP IKQELICKWI EPEQLANPKK SCNKTFSTMH

    ELVTHVTVEH VGGPEQSNHI CFWEECPREG KPFKAKYKLV NHIRVHTGEK

    PFPCPFPGCG KVFARSENLK IHKRTHTGEK PFKCEFEGCD RRFANSSDRK

    KHMHVHTSDK PYLCKMCDKS YTHPSSLRKH MKVHESSSQG SQPSPAASSG

    YESSTPPTIV SPTTDNPTTS SMSPSSSAVH HTAGHSALSS NFNEWYV

  • UCSC uc009raf.1; mouse.;



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