Human HLA-E ELISA Kit

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  • Alternative name

    Human MHC class I antigen E ELISA Kit;Human HLA-6.2 ELISA Kit;Human HLAE ELISA Kit;Human QA1 ELISA Kit;Human major histocompatibility complex, class I, E ELISA Kit;Human HLA class I histocompatibility antigen, alpha chain E ELISA Kit;

  • Catalog
    E018849
  • species
    Human
  • GeneHLA-E
  • Standard CurveHuman HLA-E ELISA Kit
  • Other Species Human HLA E ELISA KitHuman MHCE/HLA-E ELISA KitMouse HLA-E ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural humanHLA class I histocompatibility antigen, alpha chain E. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.12ng/mL.
  • Intended UseHuman HLA-E ELISA Kit allows for the in vitro quantitative determination of HLA-E , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofhumanHLA class I histocompatibility antigen, alpha chain Econcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA class I histocompatibility antigen, alpha chain E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHLA class I histocompatibility antigen, alpha chain E and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HLA class I histocompatibility antigen, alpha chain E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA class I histocompatibility antigen, alpha chain E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human HLA class I histocompatibility antigen, alpha chain E Protein information
  • Uniprot ID HLAE_HUMAN
  • Uniprot AC P13747; Q30169; Q9BT83; Q9GIY7; Q9GIY8;
  • UniGene Hs.650174;
  • GeneID 3133
  • KEGG hsa:3133;
  • Human HLA class I histocompatibility antigen, alpha chain E Protein SEQUENCE
  • SEQUENCE 358 AA; 40157 MW; 2A211B603591D248 CRC64;

    MVDGTLLLLL SEALALTQTW AGSHSLKYFH TSVSRPGRGE PRFISVGYVD

    DTQFVRFDND AASPRMVPRA PWMEQEGSEY WDRETRSARD TAQIFRVNLR

    TLRGYYNQSE AGSHTLQWMH GCELGPDRRF LRGYEQFAYD GKDYLTLNED

    LRSWTAVDTA AQISEQKSND ASEAEHQRAY LEDTCVEWLH KYLEKGKETL

    LHLEPPKTHV THHPISDHEA TLRCWALGFY PAEITLTWQQ DGEGHTQDTE

    LVETRPAGDG TFQKWAAVVV PSGEEQRYTC HVQHEGLPEP VTLRWKPASQ

    PTIPIVGIIA GLVLLGSVVS GAVVAAVIWR KKSSGGKGGS YSKAEWSDSA

    QGSESHSL




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