Human GPR109B ELISA Kit

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  • Alternative name

    Hydroxycarboxylic acid receptor 3 ELISA KIT; G-protein coupled receptor 109B ELISA KIT; G-protein coupled receptor HM74 ELISA KIT; G-protein coupled receptor HM74B ELISA KIT; Niacin receptor 2 ELISA KIT; Nicotinic acid receptor 2HCAR3 ELISA KIT; GPR109B ELISA KIT; HCA3 ELISA KIT; HM74B ELISA KIT; NIACR2 ELISA KIT

  • Catalog
  • species
  • GeneGPR109B
  • Standard CurveHuman GPR109B ELISA Kit
  • Other Species Mouse GPR109B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GPR109B. No significant cross-reactivity or interference between GPR109B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of GPR109B is typically less than 0.061ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
  • Intended UseHuman GPR109B ELISA Kit allows for the in vitro quantitative determination of GPR109B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPR109B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GPR109B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPR109B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GPR109B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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