Human HDAC3 ELISA Kit

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  • Alternative name

    Human HD3 ELISA Kit;Human RPD3-2 ELISA Kit;Human SMAP45 ELISA Kit;Human RPD3 ELISA Kit;Human histone deacetylase 3 ELISA Kit;

  • Catalog
    E018799
  • species
    Human
  • GeneHDAC3
  • Standard CurveHuman HDAC3 ELISA Kit
  • Other Species Mouse Hdac3 ELISA KitChicken HDAC3 ELISA KitRat Hdac3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HDAC3. No significant cross-reactivity or interference between HDAC3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HDAC3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman HDAC3 ELISA Kit allows for the in vitro quantitative determination of HDAC3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: HDAC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HDAC3 antibody and an HDAC3-HRP conjugate. The assay sample and buffer are incubated together with HDAC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HDAC3 concentration since HDAC3 from samples and HDAC3-HRP conjugate compete for the anti-HDAC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by HDAC3 from the sample, fewer sites are left to bind HDAC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HDAC3 concentration in each sample is interpolated from this standard curve.
  • Human Histone deacetylase 3 Protein information
  • Uniprot ID HDAC3_HUMAN
  • Uniprot AC O15379; D3DQE1; O43268; Q9UEI5; Q9UEV0;
  • UniGene Hs.519632;
  • GeneID 8841
  • KEGG hsa:8841;
  • Human Histone deacetylase 3 Protein SEQUENCE
  • SEQUENCE 428 AA; 48848 MW; 94485C1EBDCF5AD0 CRC64;

    MAKTVAYFYD PDVGNFHYGA GHPMKPHRLA LTHSLVLHYG LYKKMIVFKP

    YQASQHDMCR FHSEDYIDFL QRVSPTNMQG FTKSLNAFNV GDDCPVFPGL

    FEFCSRYTGA SLQGATQLNN KICDIAINWA GGLHHAKKFE ASGFCYVNDI

    VIGILELLKY HPRVLYIDID IHHGDGVQEA FYLTDRVMTV SFHKYGNYFF

    PGTGDMYEVG AESGRYYCLN VPLRDGIDDQ SYKHLFQPVI NQVVDFYQPT

    CIVLQCGADS LGCDRLGCFN LSIRGHGECV EYVKSFNIPL LVLGGGGYTV

    RNVARCWTYE TSLLVEEAIS EELPYSEYFE YFAPDFTLHP DVSTRIENQN

    SRQYLDQIRQ TIFENLKMLN HAPSVQIHDV PADLLTYDRT DEADAEERGP

    EENYSRPEAP NEFYDGDHDN DKESDVEI

  • UCSC uc003llf.3; human. [O15379-1];


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