Human CXCL5 ELISA Kit

Alternative name
Human ENA-78(1-78) ELISA Kit;Human epithelial-derived neutrophil-activating protein 78 ELISA Kit;Human neutrophil-activating peptide ENA-78 ELISA Kit;Human Small-inducible cytokine B5 ELISA Kit;Human ENA78 ELISA Kit;Human SCYB5 ELISA Kit;Human ENA-78 ELISA Kit;Human C-X-C motif chemokine ligand 5 ELISA Kit;Human C-X-C motif chemokine 5 ELISA Kit;Human chemokine (C-X-C motif) ligand 5 ELISA Kit;Human neutrophil-activating protein 78 ELISA Kit;Human small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) ELISA Kit;
Catalog
E010465
species
Human
GeneCXCL5
SpecificityNatural and recombinant human CXCL5
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman CXCL5 ELISA Kit allows for the in vitro quantitative determination of CXCL5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli,A37-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL5 amount of sample captured in plate. Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
Human ENA-78(9-78) Protein information
Uniprot ID CXCL5_HUMAN
Uniprot AC P42830; Q96QE1;
UniGene Hs.89714;
GeneID 6374
KEGG hsa:6374;
Human ENA-78(9-78) Protein SEQUENCE
SEQUENCE 114 AA; 11972 MW; 56B21EE86AE952D3 CRC64;
MSLLSSRAAR VPGPSSSLCA LLVLLLLLTQ PGPIASAGPA AAVLRELRCV
CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK
KVIQKILDGG NKEN

Human CXCL5 ELISA Kit

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