Human HTN5 ELISA Kit

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  • Alternative name

    Histatin-3 ELISA KIT; Basic histidine-rich protein ELISA KIT; Hst ELISA KIT; Histidine-rich protein 3 ELISA KIT; PBCleaved into the following 26 chains:Histatin-3 ELISA KIT; His3-(20-44)-peptide ELISA KIT; His3 20/44Alternative name(s):His3-(1-25)-peptide ELISA KIT; His3 1/25 ELISA KIT; Histatin-3 1/25 ELISA KIT; Histatin-6HTN3 ELISA KIT; HIS2 ELISA KIT; Hst ELISA KIT; His3 20/44 ELISA KIT; His3 1/25 ELISA KIT; His3 20/43 ELISA KIT; His3 1/24 ELISA KIT; His3 20/32 ELISA KIT; His3 1/13 ELISA KIT; His3 20/31 ELISA KIT; His3 1/12 ELISA KIT; His3 20/30 ELISA KIT; His3 1/11 ELISA KIT; His3 24/32 ELISA KIT; His3 5/13 ELISA KIT; His3 24/31 ELISA KIT; His3 5/12 ELISA KIT; His3 24/30 ELISA KIT; His3 5/11 ELISA KIT; His3 25/32 ELISA KIT; His3 6/13 ELISA KIT; His3 25/30 ELISA KIT; His3 6/11 ELISA KIT; His3 26/32 ELISA KIT; Hi ELISA KIT

  • Catalog
    E018700
  • species
    Human
  • GeneHTN5
  • Other Species Mouse HTN5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HTN5. No significant cross-reactivity or interference between HTN5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HTN5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman HTN5 ELISA Kit allows for the in vitro quantitative determination of HTN5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: HTN5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HTN5 antibody and an HTN5-HRP conjugate. The assay sample and buffer are incubated together with HTN5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HTN5 concentration since HTN5 from samples and HTN5-HRP conjugate compete for the anti-HTN5 antibody binding site. Since the number of sites is limited, as more sites are occupied by HTN5 from the sample, fewer sites are left to bind HTN5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HTN5 concentration in each sample is interpolated from this standard curve.



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