Human HIF-1alpha ELISA Kit

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  • Alternative name

    Hypoxia-inducible factor 1-alpha ELISA KIT; ARNT-interacting protein ELISA KIT; Basic-helix-loop-helix-PAS protein MOP1 ELISA KIT; Class E basic helix-loop-helix protein 78 ELISA KIT; bHLHe78 ELISA KIT; Member of PAS protein 1 ELISA KIT; PAS domain-containing protein 8HIF1A ELISA KIT; BHLHE78 ELISA KIT; MOP1 ELISA KIT; PASD8 ELISA KIT; HIF-1-alpha ELISA KIT; HIF1-alpha ELISA KIT; bHLHe78 ELISA KIT

  • Catalog
    E018574
  • species
    Human
  • GeneHIF-1alpha
  • Other Species Mouse HIF-1alpha ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human HIF-1alpha. No significant cross-reactivity or interference between Human HIF-1alpha and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseHuman HIF-1alpha ELISA Kit allows for the in vitro quantitative determination of HIF-1alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HIF-1alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HIF-1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HIF-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HIF-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HIF-1alpha. You can calculate the concentration of HIF-1alpha in the samples by comparing the OD of the samples to the standard curve.


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