Human HNRNPF ELISA Kit

Alternative name
Human hnRNP F ELISA Kit;Human nucleolin-like protein mcs94-1 ELISA Kit;Human HNRPF ELISA Kit;Human OK/SW-cl.23 ELISA Kit;Human mcs94-1 ELISA Kit;Human heterogeneous nuclear ribonucleoprotein F ELISA Kit;Human HnRNP F protein ELISA Kit;
Catalog
E018504
species
Human
GeneHNRNPF
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman HNRNPF ELISA Kit allows for the in vitro quantitative determination of HNRNPF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HNRNPF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HNRNPF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HNRNPF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HNRNPF can be calculated.
Human Heterogeneous nuclear ribonucleoprotein F, N-terminally processed Protein information
Uniprot ID HNRPF_HUMAN
Uniprot AC P52597; B3KM84; Q5T0N2; Q96AU2;
UniGene Hs.712955; Hs.808;
GeneID 3185
KEGG hsa:3185;
Human Heterogeneous nuclear ribonucleoprotein F, N-terminally processed Protein SEQUENCE
SEQUENCE 415 AA; 45672 MW; D14E170631FB1F31 CRC64;
MMLGPEGGEG FVVKLRGLPW SCSVEDVQNF LSDCTIHDGA AGVHFIYTRE
GRQSGEAFVE LGSEDDVKMA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS
GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK
ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK
FMSVQRPGPY DRPGTARRYI GIVKQAGLER MRPGAYSTGY GGYEEYSGLS
DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA
NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC
YGAGYSGQNS MGGYD
UCSC uc001jar.2; human.;

Category

Human HNRNPF ELISA Kit

Human hnRNP F ELISA Kit;Human nucleolin-like protein mcs94-1 ELISA Kit;Human HNRPF ELISA Kit;Human OK/SW-cl.23 ELISA Kit;Human mcs94-1 ELISA Kit;Human heterogeneous nuclear ribonucleoprotein F ELISA Kit;Human HnRNP F protein ELISA Kit;